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Abstract:

Underivatized carbohydrates of tulip bulb and leaf tissues were characterized in situ by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) by using carbon nanotubes (CNTs) as matrix. Two sample preparation methods-(i) depositing CNTs on the fresh tissue slices placed on the probe and (ii) locating semitransparent tissues on a dried layer of CNTs on the probe-were examined. Furthermore, practicability of in situ starch analysis by MALDI-TOF MS was examined by detection of glucose originated from on-probe amyloglucosidase-catalyzed degradation of starch on the tissue surface. Besides, CNTs could efficiently desorb/ionize natural mono-, di-, and oligosaccharides extracted from tulip bulb tissues as well as glucose resulting from starch enzymatic degradation in vitro. These results were compared with those obtained by in situ MALDI-TOF MS analysis of similar tissues. Positive ion mode showed superior signal reproducibility. CNTs deposited under semitransparent tissue could also desorb/ionize neutral carbohydrates, leading to nearly complete elimination of matrix cluster signals but with an increase in tissue-originated signals. Furthermore, several experiments were carried out to compare the efficiency of 2,5-dihydroxybenzoic acid, nor-harmane, α-cyano-4-hydroxycinnamic acid, and CNTs as matrices for MALDI of neutral carbohydrates from the intact plant tissue surface and for enzymatic tissue starch degradation; these results are discussed in brief. Among matrices studied, the lowest laser power was needed to acquire carbohydrate signals with high signal-to-noise ratio and resolution when CNTs were used. © 2008 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:In situ analysis of plant tissue underivatized carbohydrates and on-probe enzymatic degraded starch by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by using carbon nanotubes as matrix
Autor:Gholipour, Y.; Nonami, H.; Erra-Balsells, R.
Filiación:United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, 790-8566, Japan
Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, Matsuyama, 790-8566, Japan
CIHIDECAR-CONICET, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, 1428 Buenos Aires, Argentina
Palabras clave:Amyloglucosidase; CHCA; DHB; Nor-harmane; On-probe tissue analysis; Tulip; alpha cyano 4 hydroxycinnamic acid; carbohydrate; carbon nanotube; gentisic acid; glucose; harman; nor harmane; starch; unclassified drug; article; carbohydrate analysis; enzymatic degradation; glucose assay; matrix assisted laser desorption ionization time of flight mass spectrometry; molecular probe; nonhuman; plant leaf; plant tissue; priority journal; signal noise ratio; Analytic Sample Preparation Methods; Carbohydrates; Glucan 1,4-alpha-Glucosidase; Glucose; Nanotubes, Carbon; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Starch; Tulipa; Tulipa
Año:2008
Volumen:383
Número:2
Página de inicio:159
Página de fin:167
DOI: http://dx.doi.org/10.1016/j.ab.2008.08.034
Título revista:Analytical Biochemistry
Título revista abreviado:Anal. Biochem.
ISSN:00032697
CODEN:ANBCA
CAS:alpha cyano 4 hydroxycinnamic acid, 28166-41-8; gentisic acid, 490-79-9; glucose, 50-99-7, 84778-64-3; harman, 486-84-0; starch, 9005-25-8, 9005-84-9; Carbohydrates; Glucan 1,4-alpha-Glucosidase, EC 3.2.1.3; Glucose, 50-99-7; Nanotubes, Carbon; Starch, 9005-25-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v383_n2_p159_Gholipour

Referencias:

  • Karas, M., Bachmann, D., Bahr, U., Hillenkamp, F., Matrix-assisted ultraviolet laser desorption of non-volatile compounds (1987) Intl. J. Mass Spectrom. Ion Processes, 78, pp. 53-68
  • Harvey, D.J., Naven, T.J.P., Küster, B., Identification of oligosaccharides by matrix-assisted laser desorption ionization and electrospray MS (1996) Biochem. Soc. Trans., 24, pp. 905-912
  • Stahl, B., Linos, A., Karas, M., Hillenkamp, F., Steup, M., Analysis of fructans from higher plants by matrix-assisted laser desorption/ionization mass spectrometry (1997) Anal. Biochem., 246, pp. 195-204
  • Caprioli, R.M., Farmer, T.B., Gile, J., Molecular imaging of biological samples: Localization of peptides and proteins using MALDI-TOF MS (1997) Anal. Chem., 69, pp. 4751-4760
  • Chaurand, P., Stoeckli, M., Caprioli, R.M., Direct profiling of proteins in biological tissue sections by MALDI mass spectrometry (1999) Anal. Chem., 71, pp. 5263-5270
  • Peter, J., Todd, T., Schaaff, G., Chaurand, P., Caprioli, R.M., Organic ion imaging of biological tissue with secondary ion mass spectrometry and matrix-assisted laser desorption/ionization (2001) J. Mass Spectrom., 36, pp. 355-369
  • Chaurand, P., DaGue, B.B., Pearsall, R.S., Threadgill, D.W., Caprioli, R.M., Profiling proteins from azoxymethane-induced colon tumors at the molecular level by matrix-assisted laser desorption/ionization mass spectrometry (2001) Proteomics, 10, pp. 1320-1326
  • Stoeckli, M., Chaurand, P., Hallahan, D.E., Caprioli, R.M., Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues (2001) Nat. Med., 7, pp. 493-496
  • Chaurand, P., Schwartz, S.A., Billheimer, D., Xu, B.J., Crecelius, A., Caprioli, R.M., Integrating histology and imaging mass spectrometry (2004) Anal. Chem., 76, pp. 1145-1155
  • Pierson, J., Norris, J.L., Aerni, H.R., Svenningsson, P., Caprioli, R.M., Andrén, P.E.J., Molecular profiling of experimental Parkinson's disease: Direct analysis of peptides and proteins on brain tissue sections by MALDI mass spectrometry (2004) Proteom. Res., 2, pp. 289-295
  • Burrell, M., Burrell I, M., Earnshaw, C.J., Clench, M.R., Imaging matrix assisted laser desorption ionization mass spectrometry: a technique to map plant metabolites within tissues at high spatial resolution (2007) J. Exp. Bot., 58, pp. 757-763
  • Robinson, S., Warburton, K., Seymour, M., Clench, M., Thomas, J., Localization of water-soluble carbohydrates in wheat stems using imaging matrix-assisted laser desorption ionization mass spectrometry (2007) New Phytol., 173, pp. 438-444
  • Zhang, H., Cha, S., Yeung, E.S., Colloidal graphite-assisted laser desorption/ionization MS and MSn of small molecules: II. Direct profiling and MS imaging of small metabolites from fruits (2007) Anal. Chem., 79, pp. 6575-6584
  • Sluszny, C., Yeung, E.S., Nikolau, B.J., In-situ probing of the biotic-abiotic boundary of plants by laser desorption/ionization time-of-flight mass spectrometry (2005) J. Am. Soc. Mass Spectrom., 16, pp. 107-115
  • Wu, W., Liang, Z., Zhao, Z., Cai, Z., Direct analysis of alkaloid profiling in plant tissue by using matrix-assisted laser desorption/ionization mass spectrometry (2007) J. Mass Spectrom., 42, pp. 58-69
  • Ng, K.M., Liang, Z., Lu, W., Tang, H.W., Zhao, Z., Che, C.M., Cheng, Y.C., In vivo analysis and spatial profiling of phytochemicals in herbal tissue by matrix-assisted laser desorption/ionization mass spectrometry (2007) Anal. Chem., 79, pp. 2745-2755
  • Kinumi, T., Saisu, T., Takayama, M., Niwa, H., Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using an inorganic particle matrix for small molecule analysis (2000) J. Mass Spectrom., 35, pp. 417-422
  • Harvey, D.J., Matrix-assisted laser desorption/ionization mass spectrometry of carbohydrates (1999) Mass Spectrom. Rev., 18, pp. 349-451
  • Harvey, D.J., Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update covering the period 1999-2000 (2006) Mass Spectrom. Rev., 25, pp. 595-662
  • Harvey, D.J., Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update covering the period 2001-2002 (2008) Mass Spectrom. Rev., 27, pp. 125-201
  • Nonami, H., Fukui, S., Erra-Balsells, R., Β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-fight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles, and classical matrices (1997) J. Mass Spectrom., 32, pp. 287-296
  • Ren, S., Zhang, L., Cheng, Z., Guo, Y., Immobilized carbon nanotubes as matrix for MALDI-TOF-MS analysis: Applications to neutral small carbohydrates (2005) J. Am. Soc. Mass Spectrom., 16, pp. 333-339
  • Wada, H., Iwaya-Inoue, M., Akita, M., Nonami, H., Hydraulic conductance associated with growth of flower stalks, leaves, and roots in tulip plants (2004) Environ. Control Biol., 42, pp. 193-203
  • Wada, H., Iwaya-Inoue, M., Akita, M., Nonami, H., Direct measurements of cell turgor and hydraulic conductance in expanding tulip tepals (2004) Environ. Control Biol., 42, pp. 205-215
  • Wada, H., Iwaya-Inoue, M., Akita, M., Nonami, H., Hydraulic conductance in tepal growth and extension of vase life with trehalose in cut tulip flowers (2005) J. Am. Soc. Horticult. Sci., 130, pp. 275-286
  • Moe, R., Wickstrom, A., The effect of storage temperature on shoot growth, flowering, and carbohydrate metabolism in tulip bulbs (1973) Physiol. Plant., 28, pp. 81-87
  • Ho, L.C., Rees, A.R., Aspects of translocation of carbon in the tulip (1975) New Phytol., 74, pp. 421-428
  • Lambrechts, H., Rook, F., Kolloffell, C., Carbohydrate status of tulip bulbs during cold-induced flower stalk elongation and flowering (1994) Plant Physiol., 10, pp. 515-520
  • Manners, D.J., Starch (1985) Biochemistry of Storage Carbohydrates in Green Plants, pp. 149-203. , Dey P.M., and Dixon R.A. (Eds), Academic Press, San Diego
  • Zumbuhl, S., Knochenmuss, R., Wulfert, S., Dubois, F., Dale, M.J., Zenobi, R.A., Graphite-assisted laser desorption/ionization study of light-induced aging in triterpene dammar and mastic varnishes (1998) Anal. Chem., 70, pp. 707-715
  • Ugarov, M.V., Egan, T., Khabashesku, D.V., Schultz, J.A., Peng, H.Q., Khabashesku, V.N., Furutani, H., Woods, A.S., MALDI matrices for biomolecular analysis based on functionalized carbon nanomaterials (2004) Anal. Chem., 76, pp. 6734-6742
  • Xu, S.Y., Li, Y.F., Zou, H.F., Qiu, J.S., Guo, Z., Guo, B.C., Carbon nanotubes as assisted matrix for laser desorption/ionization time-of-flight mass spectrometry (2003) Anal. Chem., 75, pp. 6191-6195
  • Black, C., Poile, C., Langley, J., Herniman, J., The use of pencil lead as a matrix and calibrant for matrix-assisted laser desorption/ionization (2006) J. Rapid Commun. Mass Spectrom., 20, pp. 1053-1060
  • Mesaros, M., Tarzi, O.I., Erra-Balsells, R., Bilmes, G.M., The photophysics of some UV-MALDI matrices studied by using spectroscopic, photoacoustic, and luminescence techniques (2006) Chem. Phys. Lett., 426, pp. 334-340
  • O. I. Tarzi, H. Nonami, R. Erra-Balsells, Temperature effect on the stability of compounds used as UV-MALDI-MS matrix: 2,5-Dihydroxybenzoic acid, 2,4,6-trihydroxyacetophenone, trans-α-cyano-4-hydroxycinnamic acid, nor-harmane, and harmane, J. Mass Spectrom. (submitted for publication)

Citas:

---------- APA ----------
Gholipour, Y., Nonami, H. & Erra-Balsells, R. (2008) . In situ analysis of plant tissue underivatized carbohydrates and on-probe enzymatic degraded starch by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by using carbon nanotubes as matrix. Analytical Biochemistry, 383(2), 159-167.
http://dx.doi.org/10.1016/j.ab.2008.08.034
---------- CHICAGO ----------
Gholipour, Y., Nonami, H., Erra-Balsells, R. "In situ analysis of plant tissue underivatized carbohydrates and on-probe enzymatic degraded starch by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by using carbon nanotubes as matrix" . Analytical Biochemistry 383, no. 2 (2008) : 159-167.
http://dx.doi.org/10.1016/j.ab.2008.08.034
---------- MLA ----------
Gholipour, Y., Nonami, H., Erra-Balsells, R. "In situ analysis of plant tissue underivatized carbohydrates and on-probe enzymatic degraded starch by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by using carbon nanotubes as matrix" . Analytical Biochemistry, vol. 383, no. 2, 2008, pp. 159-167.
http://dx.doi.org/10.1016/j.ab.2008.08.034
---------- VANCOUVER ----------
Gholipour, Y., Nonami, H., Erra-Balsells, R. In situ analysis of plant tissue underivatized carbohydrates and on-probe enzymatic degraded starch by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by using carbon nanotubes as matrix. Anal. Biochem. 2008;383(2):159-167.
http://dx.doi.org/10.1016/j.ab.2008.08.034