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Abstract:

We have developed a system that allows focal drug application for cell culture microscopy. Single-cell drug delivery is achieved through the insertion of a patch-clamp-like micropipette in a microenvironment-controlled chamber mounted on a standard 35-mm Petri dish. The system has precise control of temperature, CO2 concentration, and humidity, while preventing contamination during experiments. The use of standard Petri dishes allows long-term experiments by alternating in situ microscopy with incubator growth. Modern biological long-term experiments such as the characterization of drug effects on cell movement, axonal guidance, mitosis, apoptosis, differentiation, or volume regulation can be performed. The chamber is compatible with any inverted microscope without significant modifications. © 2007 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Encapsulated Petri dish system for single-cell drug delivery and long-term time lapse microscopy
Autor:Salierno, M.; Cabrera, R.; Filevich, O.; Etchenique, R.
Filiación:Departamento de Química Inorgánica, Analítica y Química Física, INQUIMAE, Facultad de Ciencias Exactas y Naturales, C1428EHA Buenos Aires, Argentina
Palabras clave:Culture chamber; Drug delivery; Long term; Real time; Single-cell manipulation; Time-lapse microscopy; carbon dioxide; animal cell; apoptosis; article; cell differentiation; cell motility; cell strain BHK; concentration (parameters); humidity; micropipette; microscope; microscopy; mitosis; nerve fiber growth; newborn; nonhuman; patch clamp; priority journal; regulatory mechanism; standard; temperature; Animals; Cells, Cultured; Cricetinae; Drug Delivery Systems; Equipment Design; Microscopy, Video; Time Factors
Año:2007
Volumen:371
Número:2
Página de inicio:208
Página de fin:214
DOI: http://dx.doi.org/10.1016/j.ab.2007.08.005
Título revista:Analytical Biochemistry
Título revista abreviado:Anal. Biochem.
ISSN:00032697
CODEN:ANBCA
CAS:carbon dioxide, 124-38-9, 58561-67-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v371_n2_p208_Salierno

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Citas:

---------- APA ----------
Salierno, M., Cabrera, R., Filevich, O. & Etchenique, R. (2007) . Encapsulated Petri dish system for single-cell drug delivery and long-term time lapse microscopy. Analytical Biochemistry, 371(2), 208-214.
http://dx.doi.org/10.1016/j.ab.2007.08.005
---------- CHICAGO ----------
Salierno, M., Cabrera, R., Filevich, O., Etchenique, R. "Encapsulated Petri dish system for single-cell drug delivery and long-term time lapse microscopy" . Analytical Biochemistry 371, no. 2 (2007) : 208-214.
http://dx.doi.org/10.1016/j.ab.2007.08.005
---------- MLA ----------
Salierno, M., Cabrera, R., Filevich, O., Etchenique, R. "Encapsulated Petri dish system for single-cell drug delivery and long-term time lapse microscopy" . Analytical Biochemistry, vol. 371, no. 2, 2007, pp. 208-214.
http://dx.doi.org/10.1016/j.ab.2007.08.005
---------- VANCOUVER ----------
Salierno, M., Cabrera, R., Filevich, O., Etchenique, R. Encapsulated Petri dish system for single-cell drug delivery and long-term time lapse microscopy. Anal. Biochem. 2007;371(2):208-214.
http://dx.doi.org/10.1016/j.ab.2007.08.005