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Abstract:

The O-linked N-acetylglucosamine oligosaccharides in the mucins of Trypanosoma cruzi may contain galactofuranose or galactopyranose, depending on the strain, one of the components being the disaccharide. Since galactofuranose is a site for antibody recognition, it is desirable to have a sensitive method for the detection of the galactofuranosyl structures. In this paper, we present procedures for the separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the corresponding alditols by high-pH anion-exchange chromatography with pulse amperometric detection. All the isomeric disaccharides could be resolved on a CarboPac PA-10 column, the galactofuranose-containing disaccharides being more retained in the column. GlcNAcol and Galfβ1→4(Galpβ1→6)GlcNAcol could be analyzed in the same run. The compounds could also be separated by thin-layer chromatography on silica gel 60, a convenient method for analysis of the radio-labeled alditols obtained by reductive β-elimination in the presence of NaB3H4. Both methods were applied for the analysis of the O-linked sugars in the mucins of T. cruzi CL 14 and revealed that they contained only N-acetylglucosamine and the disaccharide Galpβ1→4GlcNAc. (C) 2000 Academic Press.

Registro:

Documento: Artículo
Título:Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi
Autor:Salto, M.L.; Gallo-Rodriguez, C.; Lima, C.; De Lederkremer, R.M.
Filiación:CIHIDECAR, Depto. de Quim. Orgánica, Ciudad Universitaria, Pabellón II, 1428, Buenos Aires, Argentina
Palabras clave:alditol; mucin; n acetylglucosamine; silica gel; disaccharide; mucin; sugar alcohol; amperometry; anion exchange chromatography; article; chemical structure; isomerism; molecular recognition; nonhuman; pH; priority journal; strain difference; thin layer chromatography; Trypanosoma cruzi; animal; carbohydrate analysis; chemistry; comparative study; evaluation; ion exchange chromatography; isolation and purification; methodology; Trypanosoma; Trypanosoma cruzi; Trypanosoma cruzi; Animals; Carbohydrate Sequence; Chromatography, Ion Exchange; Chromatography, Thin Layer; Disaccharides; Evaluation Studies; Hydrogen-Ion Concentration; Mucins; Sugar Alcohols; Trypanosoma cruzi
Año:2000
Volumen:279
Número:1
Página de inicio:79
Página de fin:84
DOI: http://dx.doi.org/10.1006/abio.1999.4466
Título revista:Analytical Biochemistry
Título revista abreviado:Anal. Biochem.
ISSN:00032697
CODEN:ANBCA
CAS:n acetylglucosamine, 7512-17-6; silica gel, 63231-67-4; Disaccharides; Mucins; Sugar Alcohols
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v279_n1_p79_Salto

Referencias:

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Citas:

---------- APA ----------
Salto, M.L., Gallo-Rodriguez, C., Lima, C. & De Lederkremer, R.M. (2000) . Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi. Analytical Biochemistry, 279(1), 79-84.
http://dx.doi.org/10.1006/abio.1999.4466
---------- CHICAGO ----------
Salto, M.L., Gallo-Rodriguez, C., Lima, C., De Lederkremer, R.M. "Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi" . Analytical Biochemistry 279, no. 1 (2000) : 79-84.
http://dx.doi.org/10.1006/abio.1999.4466
---------- MLA ----------
Salto, M.L., Gallo-Rodriguez, C., Lima, C., De Lederkremer, R.M. "Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi" . Analytical Biochemistry, vol. 279, no. 1, 2000, pp. 79-84.
http://dx.doi.org/10.1006/abio.1999.4466
---------- VANCOUVER ----------
Salto, M.L., Gallo-Rodriguez, C., Lima, C., De Lederkremer, R.M. Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi. Anal. Biochem. 2000;279(1):79-84.
http://dx.doi.org/10.1006/abio.1999.4466