Navegar

Colección

Datos Estadísticas

Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Phospholipases A1 and A2 frequently coexist in biological systems. Generation of lysophosphatidylcholine (LPC) in such systems cannot be assigned to any of these types of enzymes unless the position of the fatty acid in the lysocompound can be unambiguously determined. We here present a simple method to achieve this purpose. It is based on the initial chemical acylation of the isolated LPC with a labeled fatty acid, followed by the enzymatic analysis of the resulting phosphatidylcholine (PC), using snake or bee venom phospholipase A2. Thus, if treatment of the PC with this enzyme releases a labeled free fatty acid, it is demonstrated that the initial LPC was acylated at position sn-1, whereas if the product of hydrolysis yields labeled LPC, then the initial LPC was acylated at position sn-2. This is the first method devised to determine the source of LPC in the presence of mixtures of phospholipases A1 and A2 in complex biological systems.

Registro:

Documento: Artículo
Título:A method for distinguishing 1-acyl from 2-acyl lysophosphatidylcholines generated in biological systems
Autor:Florin-Christensen, J.; Narvaez-Vasquez, J.; Florin-Christensen, M.; Ryan, C.A.
Filiación:Dept. of Vet. Microbiol. and Pathol., Washington State University, Pullman, WA 99164-7040, United States
Inst. of Neurosci. (INEUCI-CONICET), Ciudad Universitaria, Pab. II, 4 Piso, 1428, Buenos Aires, Argentina
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, United States
Palabras clave:1-acyl-lysophosphatidylcholine; 2-acyllysophosphatidylcholine; Lysocompounds; Phospholipase A1; Phospholipase A2; Positional analysis; fatty acid; lysophosphatidylcholine; phospholipase A1; phospholipase A2; acylation; analytic method; article; enzyme assay; hydrolysis; nonhuman; priority journal
Año:1999
Volumen:276
Número:1
Página de inicio:13
Página de fin:17
DOI: http://dx.doi.org/10.1006/abio.1999.4322
Título revista:Analytical Biochemistry
Título revista abreviado:Anal. Biochem.
ISSN:00032697
CODEN:ANBCA
CAS:lysophosphatidylcholine, 93794-93-5; phospholipase A1, 9043-29-2; phospholipase A2, 9001-84-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v276_n1_p13_FlorinChristensen

Referencias:

  • Ghosh, S., Strum, J.C., Bell, R.M., (1997) FASEB J., 11, pp. 45-50
  • Dennis, E.A., Rhee, S.G., Billah, M.M., Hannun, Y.A., (1991) FASEB J., 5, pp. 2068-2077
  • Nishizuka, Y., (1995) FASEB J., 9, pp. 484-496
  • Dennis, E.A., (1997) Trends Biochem. Sci., 22, pp. 1-2
  • Leslie, C.C., (1997) J. Biol. Chem., 272, pp. 16709-16712
  • Bergey, D.R., Howe, G., Ryan, C.A., (1996) Proc. Natl. Acad. Sci. USA, 93, pp. 12053-12058
  • Franson, R., Waite, M., La Via, M., (1971) Biochemistry, 10, pp. 1942-1946
  • Pete, M.J., Ross, A.H., Exton, J.H., (1994) J. Biol. Chem., 269, pp. 19494-19500
  • Florin-Christensen, J., Florin-Christensen, M., Knudsen, J., Rasmussen, L., (1985) J. Protozool., 32, pp. 657-660
  • Florin-Christensen, J., Florin-Christensen, M., Rasmussen, L., Knudsen, J., Hansen, H.O., (1986) Comp. Biochem. Physiol., 85, pp. 149-155
  • Guberman, A., Hartmann, M., Tiedtke, A., Florin-Christensen, J., Florin-Christensen, M., (1999) J. Appl. Microbiol.
  • Hambrey, P.N., Mellors, A., Tizard, I., (1981) Mol. Biochem. Parasitol., 2, pp. 177-186
  • Eintrach, J., Maathai, R., Mellors, A., Ruben, L., (1998) Biochem. J., 336, pp. 659-666
  • Hullin-Matsuda, F., Tsujishita, Y., Nishizuka, Y., (1997) FEBS Lett., 419, pp. 117-120
  • Wolf, M.J., Gross, R.W., (1996) J. Biol. Chem., 271, pp. 30879-30885
  • Florin-Christensen, M., Florin-Christensen, J., Isola, E.D., Lammel, E., Meinardi, E., Brenner, R.R., Rasmussen, L., (1997) Mol. Biochem. Parasitol., 88, pp. 25-33
  • Dodge, J.T., Mitchell, C., Hanahan, D.J., (1963) Arch. Biochem. Biophys., 100, pp. 119-130
  • Bligh, E.G., Dyer, W.J., (1959) Can. J. Biochem. Physiol., 37, pp. 911-917
  • Ames, B.N., (1966) Methods in Enzymology, pp. 115-118. , S. P. Colowick, & N. O. Kaplan. New York: Academic Press
  • Gupta, C.M., Radhakrishnan, R., Khorana, H.G., (1977) Proc. Natl. Acad. Sci. USA, 74, pp. 4315-4319
  • Lapidot, Y., Barzilay, I., Hajdu, J., (1969) Chem. Phys. Lipids, 3, pp. 125-134
  • Zwaal, R.F.A., Fluckiger, R., Moser, S., Zahler, P., (1974) Biochim. Biophys. Acta, 373, pp. 416-424

Citas:

---------- APA ----------
Florin-Christensen, J., Narvaez-Vasquez, J., Florin-Christensen, M. & Ryan, C.A. (1999) . A method for distinguishing 1-acyl from 2-acyl lysophosphatidylcholines generated in biological systems. Analytical Biochemistry, 276(1), 13-17.
http://dx.doi.org/10.1006/abio.1999.4322
---------- CHICAGO ----------
Florin-Christensen, J., Narvaez-Vasquez, J., Florin-Christensen, M., Ryan, C.A. "A method for distinguishing 1-acyl from 2-acyl lysophosphatidylcholines generated in biological systems" . Analytical Biochemistry 276, no. 1 (1999) : 13-17.
http://dx.doi.org/10.1006/abio.1999.4322
---------- MLA ----------
Florin-Christensen, J., Narvaez-Vasquez, J., Florin-Christensen, M., Ryan, C.A. "A method for distinguishing 1-acyl from 2-acyl lysophosphatidylcholines generated in biological systems" . Analytical Biochemistry, vol. 276, no. 1, 1999, pp. 13-17.
http://dx.doi.org/10.1006/abio.1999.4322
---------- VANCOUVER ----------
Florin-Christensen, J., Narvaez-Vasquez, J., Florin-Christensen, M., Ryan, C.A. A method for distinguishing 1-acyl from 2-acyl lysophosphatidylcholines generated in biological systems. Anal. Biochem. 1999;276(1):13-17.
http://dx.doi.org/10.1006/abio.1999.4322