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Abstract:

A simple and highly sensitive method for the assay of trypsin has been developed by making use of the phosphorylated synthetic peptide Leu-Arg-Arg-Ala-Ser-(32P)-Leu-Gly as substrate. The technique has been adapted from the phosphocellulose method of R. Roskoski, Jr. (in Methods in Enzymology (Corbin, J., and Hardman, J., Eds.), Vol. 99, pp. 3-6, Academic Press, New York) used for measuring of protein kinases. In addition to measuring the activity of trypsin at the microgram level, the 32P-labeled peptide method can be used for measuring other trypsin-like enzymes. It has been successfully utilized for the identification of a new peptidase from the fungus Saccobolus platensis. © 1989.

Registro:

Documento: Artículo
Título:A radioactive method for the measurement of trypsin and trypsin-like activities
Autor:Murray, P.F.; Silberstein, S.; Cantore, M.L.; Passeron, S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 1428 Buenos Aires, Argentina
Palabras clave:peptidase; radioisotope; trypsin; enzyme assay; methodology; priority journal; radioassay; saccobolus platensis; Adenosine Triphosphate; Amino Acid Sequence; Ascomycota; Hydrogen-Ion Concentration; Peptide Hydrolases; Phosphorus Radioisotopes; Phosphorylation; Support, Non-U.S. Gov't; Trypsin; Trypsin Inhibitors
Año:1989
Volumen:179
Número:1
Página de inicio:56
Página de fin:59
DOI: http://dx.doi.org/10.1016/0003-2697(89)90199-1
Título revista:Analytical Biochemistry
Título revista abreviado:Anal. Biochem.
ISSN:00032697
CODEN:ANBCA
CAS:peptidase, 9013-14-3, 9031-96-3; trypsin, 9002-07-7; Adenosine Triphosphate, 56-65-5; Peptide Hydrolases, EC 3.4; Phosphorus Radioisotopes; Trypsin Inhibitors; Trypsin, EC 3.4.21.4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v179_n1_p56_Murray

Referencias:

  • Wolf, (1980) Adv. Microb. Physiol., 21, pp. 267-338
  • Neurath, (1984) Science, 224, pp. 350-357
  • Erlanger, Kokowsky, Cohen, (1961) Arch. Biochem. Biophys., 95, pp. 271-278
  • Lottenberg, Christensen, Jackson, Coleman, (1981) Methods in Enzymology, 80, pp. 341-361. , L. Lorand, 1st ed., Academic Press, New York
  • Glass, Masaracchia, Feramisco, Kemp, (1978) Anal. Biochem., 87, pp. 566-569
  • Roskoski, Jr., (1983) Methods in Enzymology, 99, pp. 3-6. , J. Corbin, J.D. Hardman, 1st ed., Academic Press, New York
  • Johnson, Walseth, (1979) Adv. Cyclic Nucleotide Res., 10, pp. 135-167
  • Gupta, Vijayaraj Reddy, Randerath, (1982) Carcinogenesis, 3, pp. 1081-1092
  • Bradford, (1976) Anal. Biochem., 72, pp. 248-254

Citas:

---------- APA ----------
Murray, P.F., Silberstein, S., Cantore, M.L. & Passeron, S. (1989) . A radioactive method for the measurement of trypsin and trypsin-like activities. Analytical Biochemistry, 179(1), 56-59.
http://dx.doi.org/10.1016/0003-2697(89)90199-1
---------- CHICAGO ----------
Murray, P.F., Silberstein, S., Cantore, M.L., Passeron, S. "A radioactive method for the measurement of trypsin and trypsin-like activities" . Analytical Biochemistry 179, no. 1 (1989) : 56-59.
http://dx.doi.org/10.1016/0003-2697(89)90199-1
---------- MLA ----------
Murray, P.F., Silberstein, S., Cantore, M.L., Passeron, S. "A radioactive method for the measurement of trypsin and trypsin-like activities" . Analytical Biochemistry, vol. 179, no. 1, 1989, pp. 56-59.
http://dx.doi.org/10.1016/0003-2697(89)90199-1
---------- VANCOUVER ----------
Murray, P.F., Silberstein, S., Cantore, M.L., Passeron, S. A radioactive method for the measurement of trypsin and trypsin-like activities. Anal. Biochem. 1989;179(1):56-59.
http://dx.doi.org/10.1016/0003-2697(89)90199-1