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Abstract:

A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity patterns were obtained by autoradiography or iodine staining of the gels. The method here described offers several advantages in terms of speed, sensitivity, and economy when compared with other procedures. © 1986.

Registro:

Documento: Artículo
Título:A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
Autor:Krisman, C.R.; Blumenfeld, M.L.
Filiación:Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, CONICET, Antonio Machado 151, 1405 Buenos Aires, Argentina
Palabras clave:Glycogen synthase assay; glycogen synthase gel electrophoresis; glycogen synthase polyacrylamide gel detection; iodine staining; α-1,4 glucan synthase autoradiography; α-1,4 glucan synthase iodine staining; glycogen synthase; enzyme assay; methodology; nonhuman; polyacrylamide gel electrophoresis; priority journal; alpha-Amylase; Animal; Autoradiography; Electrophoresis, Polyacrylamide Gel; Glycogen Synthase; Iodine; Myocardium; Rats; Solutions; Staining and Labeling; Support, Non-U.S. Gov't; Time Factors
Año:1986
Volumen:154
Número:2
Página de inicio:409
Página de fin:413
DOI: http://dx.doi.org/10.1016/0003-2697(86)90006-0
Título revista:Analytical Biochemistry
Título revista abreviado:Anal. Biochem.
ISSN:00032697
CODEN:ANBCA
CAS:glycogen synthase, 9033-05-0; alpha-Amylase, EC 3.2.1.1; Glycogen Synthase, EC 2.4.1.11; Iodine, 7553-56-2; Solutions
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v154_n2_p409_Krisman

Referencias:

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Citas:

---------- APA ----------
Krisman, C.R. & Blumenfeld, M.L. (1986) . A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis. Analytical Biochemistry, 154(2), 409-413.
http://dx.doi.org/10.1016/0003-2697(86)90006-0
---------- CHICAGO ----------
Krisman, C.R., Blumenfeld, M.L. "A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis" . Analytical Biochemistry 154, no. 2 (1986) : 409-413.
http://dx.doi.org/10.1016/0003-2697(86)90006-0
---------- MLA ----------
Krisman, C.R., Blumenfeld, M.L. "A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis" . Analytical Biochemistry, vol. 154, no. 2, 1986, pp. 409-413.
http://dx.doi.org/10.1016/0003-2697(86)90006-0
---------- VANCOUVER ----------
Krisman, C.R., Blumenfeld, M.L. A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis. Anal. Biochem. 1986;154(2):409-413.
http://dx.doi.org/10.1016/0003-2697(86)90006-0