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Abstract:

The high sensitivity that can be attained using a bienzymatic system and mediated by the redox polymer [Os(bpy)2 ClPyCH2NHpoly(allylamine)] (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and continuous-flow/stopped-flow/continuous-flow processing. When the hydrogen peroxide formed by LOx layer reaches the inner layer, the electronic flow between the immobilized peroxidase and the electrode surface produces a current, proportional to lactate concentration. The determination of lactate was possible with a limit of detection of 5nmoll -1 in the processing of as many as 30 samples per hour. This arrangement allows working in undiluted milk samples with a good stability and reproducibility. Horseradish peroxidase [EC 1.11.1.7] and Os-PAA were covalently immobilized on the glassy carbon electrode surface (upper cell body), lactate oxidase [EC 1.1.3.x] was immobilized on a disk that can be rotated. © 2003 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system
Autor:Torriero, A.A.J.; Salinas, E.; Battaglini, F.; Raba, J.
Filiación:Department of Analytical Chemistry, National University of San Luis, Chacabuco y Pedernera, 5700 San Luis, Argentina
INQUIMAE, University of Buenos Aires, Cd. Univ. - Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Bioreactor; Biosensors; Flow system; Horseradish peroxidase; Lactate; Lactate oxidase; Milk; Osmium; Carbon; Electrodes; Enzymes; Flow of fluids; Hydrogen peroxide; Organic compounds; Osmium; Polymers; Milk lactate determination; Bioreactors; horseradish peroxidase; hydrogen peroxide; lactate 2 monooxygenase; lactic acid; osmium derivative; polymer; article; bioreactor; continuous flow reactor; electric current; electrode; electron transport; enzyme immobilization; milk; oxidation reduction reaction; priority journal; reproducibility; rotation; Armoracia rusticana
Año:2003
Volumen:498
Número:1-2
Página de inicio:155
Página de fin:163
DOI: http://dx.doi.org/10.1016/S0003-2670(03)00897-3
Título revista:Analytica Chimica Acta
Título revista abreviado:Anal. Chim. Acta
ISSN:00032670
CODEN:ACACA
CAS:hydrogen peroxide, 7722-84-1; lactate 2 monooxygenase, 9028-72-2; lactic acid, 113-21-3, 50-21-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032670_v498_n1-2_p155_Torriero

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Citas:

---------- APA ----------
Torriero, A.A.J., Salinas, E., Battaglini, F. & Raba, J. (2003) . Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system. Analytica Chimica Acta, 498(1-2), 155-163.
http://dx.doi.org/10.1016/S0003-2670(03)00897-3
---------- CHICAGO ----------
Torriero, A.A.J., Salinas, E., Battaglini, F., Raba, J. "Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system" . Analytica Chimica Acta 498, no. 1-2 (2003) : 155-163.
http://dx.doi.org/10.1016/S0003-2670(03)00897-3
---------- MLA ----------
Torriero, A.A.J., Salinas, E., Battaglini, F., Raba, J. "Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system" . Analytica Chimica Acta, vol. 498, no. 1-2, 2003, pp. 155-163.
http://dx.doi.org/10.1016/S0003-2670(03)00897-3
---------- VANCOUVER ----------
Torriero, A.A.J., Salinas, E., Battaglini, F., Raba, J. Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system. Anal. Chim. Acta. 2003;498(1-2):155-163.
http://dx.doi.org/10.1016/S0003-2670(03)00897-3