Artículo
Lapierre, A.V.; Battaglini, F.; Raba, J. "Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples" (2001) Analytica Chimica Acta. 443(1):17-24
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Abstract:
A recently introduced redox osmium complex of the type [Os(bpy)2Cl(pyX]+, where pyX corresponds to a pyridine derivative bearing an aldehyde group, and glucose oxidase [EC 1.1.3.4] were covalently immobilized on the glassy carbon electrode surface (upper cell body). This approach has been designed in a sealed cell to be used under continuous-flow/stopped-flow/continuous-flow processing. The operating characteristic of the integrated bioreactor/detector unit has been utilized in the determination of glucose in serum samples using the electroactive polymer as mediator in the re-oxidation of GOx and amperometric monitoring. The determinations are based on the rate of response under stopped-flow conditions. The trend in values of the apparent Michaelis-Menten constant confirms the validity of the approach to sensitive determination utilizing very small amounts of biocatalysts. The determination of glucose was possible with a limit of detection of 0.020 mM in the processing of as many as 30 samples per hour. © 2001 Elsevier Science B.V. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples |
| Autor: | Lapierre, A.V.; Battaglini, F.; Raba, J. |
| Filiación: | Department of Analytical Chemistry, National University of San Luis, Chacabuco y Pedernera, 5700 San Luis, Argentina INQUIMAE, University of Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina |
| Palabras clave: | Bioreactor; Biosensors; Flow system; Osmium; aldehyde; blood extract; carbon; dianisidine; functional group; glucose; glucose oxidase; osmium derivative; polymer; pyridine derivative; amperometry; article; biocatalyst; bioreactor; blood sampling; carbohydrate analysis; cell volume; comparative study; electrode; electron transport; flow measurement; glucose assay; glucose blood level; Michaelis constant; oxidation; oxidation reduction reaction; pH; priority journal; reaction time; validation process |
| Año: | 2001 |
| Volumen: | 443 |
| Número: | 1 |
| Página de inicio: | 17 |
| Página de fin: | 24 |
| DOI: | http://dx.doi.org/10.1016/S0003-2670(01)01186-2 |
| Título revista: | Analytica Chimica Acta |
| Título revista abreviado: | Anal. Chim. Acta |
| ISSN: | 00032670 |
| CODEN: | ACACA |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032670_v443_n1_p17_Lapierre |
Referencias:
- Schuhmann, W., (1995) Mikrochim. Acta, 121, pp. 1-29
- Ghindilis, A.L., Atasanov, P., Wilkins, E., (1997) Electroanalysis, 9, pp. 661-674
- Raba, J., Mottola, H.A., (1995) Crit. Rev. Anal. Chem., 25 (1), pp. 1-42
- Bartlett, P.N., Tebbut, P., Whitaker, R.P., (1991) Progr. React. Kinet., 16, pp. 55-65
- Albery, W.J., Bartlett, N.P., Cass, A.E.G., Craston, D.H., Haggett, B.G.D., (1986) J. Chem. Soc., Faraday Trans., 82, pp. 1033-1058
- Matsumoto, K., Baeza Baeza, J.J., Mottola, H.A., (1993) Anal. Chem., 65, pp. 636-639
- Danilowicz, C., Cortón, E., Battaglini, F., (1998) J. Electroanal. Chem., 445, pp. 89-94
- Heller, A., (1992) J. Phys. Chem., 96, pp. 3579-3587
- Degani, Y., Heller, A., (1987) J. Phys. Chem., 91, pp. 1285-1289
- Degani, Y., Heller, A., (1988) J. Am. Chem. Soc., 110, pp. 2615-2620
- Heller, A., Degani, Y., (1988) Redox Chemistry and Interfacial Behavior of Biological Molecules, pp. 151-171. , G. Dryhurst, K. Niki (Eds.), Plenum Press, New York
- Lapierre, A.V., Olsina, R.A., Raba, J., (1998) Anal. Chem., 70, pp. 3679-3684
- Lapierre, A.V., Olsina, R.A., Raba, J., (1999) Anal. Chim. Acta, 396, pp. 143-149
- Opekar, F., Beran, P., (1976) J. Electroanal. Chem. Interf. Electrochem., 69, pp. 1-105
- Kamin, R.A., Wilson, G., (1980) Anal. Chem., 52, pp. 1198-1205
- Wang, J., Lin, M.S., (1998) Anal. Chem., 218, pp. 281-290
- Richter, P., López Ruiz, B., Sánchez-Cabezudo, M., Mottola, H.A., (1996) Anal. Chem., 68, pp. 1701-1705
- Briggs, E.G., Haldane, J.B.S., (1925) Biochem. J., 19, pp. 338-339
- Alcock, S.J., Turner, A.P.F., (1994) IEEE Engineering in Medicine and Biology, pp. 319-325. , June/July
Citas:
---------- APA ----------
Lapierre, A.V., Battaglini, F. & Raba, J. (2001) . Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples. Analytica Chimica Acta, 443(1), 17-24.http://dx.doi.org/10.1016/S0003-2670(01)01186-2
---------- CHICAGO ----------
Lapierre, A.V., Battaglini, F., Raba, J. "Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples" . Analytica Chimica Acta 443, no. 1 (2001) : 17-24.http://dx.doi.org/10.1016/S0003-2670(01)01186-2
---------- MLA ----------
Lapierre, A.V., Battaglini, F., Raba, J. "Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples" . Analytica Chimica Acta, vol. 443, no. 1, 2001, pp. 17-24.http://dx.doi.org/10.1016/S0003-2670(01)01186-2
---------- VANCOUVER ----------
Lapierre, A.V., Battaglini, F., Raba, J. Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples. Anal. Chim. Acta. 2001;443(1):17-24.http://dx.doi.org/10.1016/S0003-2670(01)01186-2
