Artículo

Alcaraz, M.R.; Morzán, E.; Sorbello, C.; Goicoechea, H.C.; Etchenique, R. "Multiway analysis through direct excitation-emission matrix imaging" (2018) Analytica Chimica Acta. 1032:32-39
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Abstract:

In this work, a direct in-flow methodology for the acquisition of excitation-emission fluorescence matrices is presented. The system is particularly suited for measurements in the order of tens of milliseconds. A light source operated in continuous mode is dispersed through a grating and focused onto a square-section capillary. Under the spatially resolved excitation, the emission is collected, dispersed through a second grating and further focused onto a CCD array sensor. To allow the wavelength accuracy, a spectral calibration was performed registering the scattering signal of a dispersive element using interference filters ranging from 340 nm to 740 nm. The theoretical performance of the method was analyzed and second-order data obtained for different analyte mixtures are presented and discussed. PARAFAC was applied to evaluate the trilinearity of the obtained data. Mathematical evaluation by means of the criterion of similarity corroborates the agreement between experimental pure spectra and spectral profiles retrieved from PARAFAC. Moreover, the feasibility of the spectrometer to obtain second-order data for analyses with quantitative aims was demonstrated. Finally, fast data acquisition was proved by monitoring a chromatographic analysis of dye mixtures for the generation of third-order LC-EEM data. Here, an improvement in the resolution of the different instrumental modes was demonstrated. © 2018 Elsevier B.V.

Registro:

Documento: Artículo
Título:Multiway analysis through direct excitation-emission matrix imaging
Autor:Alcaraz, M.R.; Morzán, E.; Sorbello, C.; Goicoechea, H.C.; Etchenique, R.
Filiación:Departamento de Química Inorgánica, Analítica y Química Física, INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, Pabellón 2, Buenos Aires, C1428EGA, Argentina
Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe, S3000ZAA, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz, 2290, Buenos Aires, C1425FQB, Argentina
Comisión Nacional de Energía Atómica, General Paz 1499, Buenos Aires, B1650KNA, Argentina
Palabras clave:Chemometrics; Excitation-emission matrix; Higher-order data analysis; Imaging; Charge coupled devices; Chromatographic analysis; Data acquisition; Imaging techniques; Light sources; Mixtures; Chemometrics; Excitation emission fluorescence; Excitation emission matrices; Fast data acquisition; Higher-order; Interference filters; Spectral calibration; Theoretical performance; Matrix algebra; accuracy; algorithm; Article; calculation; calibration; excitation emission matrix imaging; image analysis; imaging; light; liquid chromatography; luminance; mathematical analysis; measurement; Parallel factor analysis; priority journal; reliability; validity
Año:2018
Volumen:1032
Página de inicio:32
Página de fin:39
DOI: http://dx.doi.org/10.1016/j.aca.2018.07.069
Título revista:Analytica Chimica Acta
Título revista abreviado:Anal. Chim. Acta
ISSN:00032670
CODEN:ACACA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032670_v1032_n_p32_Alcaraz

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Citas:

---------- APA ----------
Alcaraz, M.R., Morzán, E., Sorbello, C., Goicoechea, H.C. & Etchenique, R. (2018) . Multiway analysis through direct excitation-emission matrix imaging. Analytica Chimica Acta, 1032, 32-39.
http://dx.doi.org/10.1016/j.aca.2018.07.069
---------- CHICAGO ----------
Alcaraz, M.R., Morzán, E., Sorbello, C., Goicoechea, H.C., Etchenique, R. "Multiway analysis through direct excitation-emission matrix imaging" . Analytica Chimica Acta 1032 (2018) : 32-39.
http://dx.doi.org/10.1016/j.aca.2018.07.069
---------- MLA ----------
Alcaraz, M.R., Morzán, E., Sorbello, C., Goicoechea, H.C., Etchenique, R. "Multiway analysis through direct excitation-emission matrix imaging" . Analytica Chimica Acta, vol. 1032, 2018, pp. 32-39.
http://dx.doi.org/10.1016/j.aca.2018.07.069
---------- VANCOUVER ----------
Alcaraz, M.R., Morzán, E., Sorbello, C., Goicoechea, H.C., Etchenique, R. Multiway analysis through direct excitation-emission matrix imaging. Anal. Chim. Acta. 2018;1032:32-39.
http://dx.doi.org/10.1016/j.aca.2018.07.069