Artículo

Víctor, M.; Ruiz-Henestrosa, P.; Martinez, M.J.; Patino, J.M.R.; Pilosof, A.M.R. "A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions" (2012) JAOCS, Journal of the American Oil Chemists' Society. 89(7):1183-1191
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Abstract:

In this work, the complex assembly of one of the major storage proteins in soybean, glycinin, was analyzed using dynamic light scattering, from the hydrodynamic diameter of assembled forms in solution. The protein concentration and temperature were maintained constant at 10-1% w/w and 20 °C, respectively, and the pH was 7.6, 7.0 and 3.0. By analyzing the intensity and volume size distributions, a complex equilibrium between self-assembled forms could be determined. At pH 7.6 and an ionic strength of 0.5 M, where the self-assembly of glycinin has been widely reported in the literature, the DLS technique revealed an equilibrium between different assembled forms, that shifted towards the 11S form. At a lower ionic strength for pH 3.0 or 7.0, the 7S form predominated. The hydrodynamic diameter evolved differently upon heating, depending on pH and ionic strength. For pH 7 (I = 0.05) and 7.6 (I = 0.5) a significant increase in d H was observed at a temperatures of 55 and 70 °C, respectively, which were significantly lower than the denaturation onset temperatures as determined by DSC. No changes in dH nor a transition endotherm were observed at pH 3. © 2012 AOCS.

Registro:

Documento: Artículo
Título:A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions
Autor:Víctor, M.; Ruiz-Henestrosa, P.; Martinez, M.J.; Patino, J.M.R.; Pilosof, A.M.R.
Filiación:Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria Avda, Intendente Guiraldes s/n, 1428 Buenos Aires, Argentina
Departamento de Ingenieria Quimica, Facultad de Quimica, Universidad de Sevilla, c/Profesor Garcia Gonzalez, 1, 41012 Sevilla, Spain
Palabras clave:Dynamic light scattering; Globulin; Soy; Structure; Complex assembly; Complex equilibria; Globulin; Glycinin; Hydrodynamic diameter; Onset temperature; Protein concentrations; Self-assembled; Soy; Soy globulin; Storage proteins; Volume size distribution; Hydrodynamics; Ionic strength; Structure (composition); Dynamic light scattering; Glycine max
Año:2012
Volumen:89
Número:7
Página de inicio:1183
Página de fin:1191
DOI: http://dx.doi.org/10.1007/s11746-012-2029-7
Título revista:JAOCS, Journal of the American Oil Chemists' Society
Título revista abreviado:JAOCS J Am Oil Chem Soc
ISSN:0003021X
CODEN:JAOCA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0003021X_v89_n7_p1183_Victor

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Citas:

---------- APA ----------
Víctor, M., Ruiz-Henestrosa, P., Martinez, M.J., Patino, J.M.R. & Pilosof, A.M.R. (2012) . A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions. JAOCS, Journal of the American Oil Chemists' Society, 89(7), 1183-1191.
http://dx.doi.org/10.1007/s11746-012-2029-7
---------- CHICAGO ----------
Víctor, M., Ruiz-Henestrosa, P., Martinez, M.J., Patino, J.M.R., Pilosof, A.M.R. "A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions" . JAOCS, Journal of the American Oil Chemists' Society 89, no. 7 (2012) : 1183-1191.
http://dx.doi.org/10.1007/s11746-012-2029-7
---------- MLA ----------
Víctor, M., Ruiz-Henestrosa, P., Martinez, M.J., Patino, J.M.R., Pilosof, A.M.R. "A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions" . JAOCS, Journal of the American Oil Chemists' Society, vol. 89, no. 7, 2012, pp. 1183-1191.
http://dx.doi.org/10.1007/s11746-012-2029-7
---------- VANCOUVER ----------
Víctor, M., Ruiz-Henestrosa, P., Martinez, M.J., Patino, J.M.R., Pilosof, A.M.R. A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions. JAOCS J Am Oil Chem Soc. 2012;89(7):1183-1191.
http://dx.doi.org/10.1007/s11746-012-2029-7