Artículo

Longhi, S.A.; Brandariz, S.B.; Lafon, S.O.; Niborski, L.L.; Luquetti, A.O.; Schijman, A.G.; Levin, M.J.; Gómez, K.A. "Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms" (2012) American Journal of Tropical Medicine and Hygiene. 87(2):267-271
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Abstract:

The aim of this work was to investigate the potential usefulness of Trypanosoma cruzi lysate, recombinant protein JL7, and peptides P013, R13, JL18, JL19, and P0b as serological markers for human Chagas disease. We analyzed 228 sera from Brazilian Chagas disease patients classified into four clinical groups and 108 from non-chagasic patients. We defined the diagnostic sensitivity, specificity, and Kappa index measured by enzyme-linked immunosorbent assay (ELISA). As previously described, the highest values of diagnostic parameters were achieved for T. cruzi lysate and JL7; peptide P013 showed high specificity but low sensitivity. The other peptides resulted in lower sensitivity and specificity in our ELISA than T. cruzi lysate and JL7 protein. Antibodies against JL7 protein were mainly detected in sera from patients with severe chagasic cardiomyopathy, compared with those from the indeterminate form, whereas peptides failed to discriminate between the clinical forms of the disease. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.

Registro:

Documento: Artículo
Título:Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms
Autor:Longhi, S.A.; Brandariz, S.B.; Lafon, S.O.; Niborski, L.L.; Luquetti, A.O.; Schijman, A.G.; Levin, M.J.; Gómez, K.A.
Filiación:INGEBI - CONICET, Buenos Aires, Argentina
Instituto de Patologia Tropical e Saude Publica - Universidad Federal de Goias, Goiania, Brazil
Vuelta de Obligado 2490, Buenos Aires 1428, Argentina
Palabras clave:bacterium lysate; peptide; peptide jl18; peptide jl19; peptide p013; peptide p0beta; peptide r13; recombinant antigen; recombinant protein; recombinant protein jl7; unclassified drug; adolescent; adult; article; blood sampling; Brazil; Chagas disease; child; controlled study; cross reaction; disease severity; enzyme linked immunosorbent assay; female; human; immunofluorescence; major clinical study; male; school child; sensitivity and specificity; serology; Trypanosoma cruzi; visceral leishmaniasis; Adolescent; Adult; Animals; Antibodies, Protozoan; Antigens, Protozoan; Brazil; Chagas Disease; Child; Enzyme-Linked Immunosorbent Assay; Female; Humans; Male; Middle Aged; Recombinant Proteins; Sensitivity and Specificity; Trypanosoma cruzi; Young Adult
Año:2012
Volumen:87
Número:2
Página de inicio:267
Página de fin:271
DOI: http://dx.doi.org/10.4269/ajtmh.2012.11-0533
Título revista:American Journal of Tropical Medicine and Hygiene
Título revista abreviado:Am. J. Trop. Med. Hyg.
ISSN:00029637
CODEN:AJTHA
CAS:bacterium lysate, 88402-38-4; Antibodies, Protozoan; Antigens, Protozoan; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v87_n2_p267_Longhi

Referencias:

  • (2006) Estimación Cuantitativa de la Enfermedad de Chagas en Las Américas, , PAHO-WHO, OPS/HDM/CD/425-06
  • Control of Chagas disease: Second Report of the WHO Expert Committee (2002) WHO Technical Report Series 905, , World Health Organization, Geneva:WHO
  • Muniz, J., Freitas, G., Contribuicao para o diagnostico da doenca de Chagas pelas reaco es de imunidade. II - Isolamento de polissacarideos de Schizotrypanum cruzi e de outros tripanosomideos, seu comportamento nas reaco es de precipitacao, de fixacao do complemento e de hipersensibilidade. Os "Tests"de floculacao (sublimado e formol-gel) (1944) Rev Bras Biol, 4, pp. 421-438
  • Cerisola, J.A., Rosenbaum, M.B., Complement-fixation reaction in the diagnosis of Chagas' disease (1958) Prensa Med Argent, 45, pp. 1454-1463
  • Alvarez, M., Cerisola, J.A., Rohweder, R.W., Test de inmunofluorescencia para el diagnóstico de la enfermedad de Chagas (1968) Bol Chil Parasitol, 23, pp. 4-8
  • Cerisola, J.A., Fatala Chaben, M., Lazzari, J.O., Hemagglutination test for the diagnosis of Chagas's disease (1962) Prensa Med Argent, 49, pp. 1761-1767
  • Vattuone, N.H., Yanovsky, J.F., Trypanosoma cruzi: Agglutination activity of enzyme-treated epimastigotes (1971) Exp Parasitol, 30, pp. 349-355
  • Voller, A., Draper, C., Bidwell, D.E., Bartlett, A., A microplate enzyme-linked immunosorbent assay (ELISA) for Chagas disease (1975) Lancet, 1, pp. 426-429
  • Cura, E.N., Ruiz, A.M., Velazquez, E., Malagrino, N., Orn, A., Segura, E.L., Estandarización de un kit de confirmación (FATALAKIT) para el inmunodiagnóstico de la infección por el Trypanosoma cruzi (1993) Medicina (B Aires), 53, p. 82
  • Caballero, Z., Sousa, O., Marques, W., Saez-Alquezar, A., Umezawa, S., Evaluation of serological tests to identify Trypanosoma cruzi infection in humans and determinate cross-reactivity with Trypanosoma rangeli and Leishmania spp (2007) Clin Vaccine Immunol, 14, pp. 1045-1049
  • Da, S.J.F., Umezawa, E.S., Luquetti, A.O., Chagas disease: Recombinant Trypanosoma cruzi antigens for serological diagnosis (2001) Trends in Parasitology, 17 (6), pp. 286-291. , DOI 10.1016/S1471-4922(01)01897-9, PII S1471492201018979
  • Silva, E.D., Pereira, V.R.A., Gomes, J.A.S., Lorena, V.M.B., Cancado, J.R., Ferreira, A.G.P., Krieger, M.A., Gomes, Y.M., Use of the EIE-Recombinant-Chagas-Biomanguinhos kit to monitor cure of human Chagas' disease (2002) Journal of Clinical Laboratory Analysis, 16 (3), pp. 132-136. , DOI 10.1002/jcla.10028
  • Umezawa, E.S., Luquetti, A.O., Levitus, G., Ponce, C., Ponce, E., Henriquez, D., Revollo, S., Da, S.J.F., Serodiagnosis of Chronic and Acute Chagas' Disease with Trypanosoma cruzi Recombinant Proteins: Results of a Collaborative Study in Six Latin American Countries (2004) Journal of Clinical Microbiology, 42 (1), pp. 449-452. , DOI 10.1128/JCM.42.1.449-452.2004
  • Chang, C.-D., Cheng, K.Y., Jiang, L.X., Salbilla, V.A., Haller, A.S., Yem, A.W., Bryant, J.D., Shah, D.O., Evaluation of a prototype Trypanosoma cruzi antibody assay with recombinant antigens on a fully automated chemiluminescence analyzer for blood donor screening (2006) Transfusion, 46 (10), pp. 1737-1744. , DOI 10.1111/j.1537-2995.2006.00965.x
  • Cooley, G., Etheridge, R.D., Boehlke, C., Bundy, B., Weatherly, D.B., Minning, T., Haney, M., Tarleton, R.L., High throughput selection of effective serodiagnostics for Trypanosoma cruzi infection (2008) PLOS Neg Trop Dis, 2, pp. 1-12
  • Luquetti, A.O., Ponce, C., Ponce, E., Esfandiari, J., Schijman, A., Revollo, S., Anez, N., Da, S.J.F., Chagas' disease diagnosis: A multicentric evaluation of Chagas Stat-Pak, a rapid immunochromatographic assay with recombinant proteins of Trypanosoma cruzi (2003) Diagnostic Microbiology and Infectious Disease, 46 (4), pp. 265-271. , DOI 10.1016/S0732-8893(03)00051-8
  • Ponce, C., Ponce, E., Vinelli, E., Montoya, A., De Aguilar, V., Gonzalez, A., Zingales, B., Da, S.J.F., Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America (2005) Journal of Clinical Microbiology, 43 (10), pp. 5065-5068. , DOI 10.1128/JCM.43.10.5065-5068.2005
  • Sosa-Estani, S., Gamboa-León, M.R., Del Cid-Lemus, J., Althabe, F., Alger, J., Almendares, O., Cafferata, M.L., Buekens, P., Use of a rapid test on umbilical cord blood to screen for Trypanosoma cruzi infection in pregnant women in Argentina, Bolivia, Honduras and Mexico (2008) Am J Trop Med Hyg, 79, pp. 755-759. , Working Group
  • (2005) Report of the Scientific Working Group on Chagas Disease, , http://apps.who.int/tdr/publications/tdr-research-publications/ reporte-enfermedad-chagas/pdf/swg_chagas.pdf, Buenos Aires, Argentina, 17-20 April Geneva: World Health Organization. Available at
  • Levin, M.J., Franco Da Silveira, J., Frasch, A.C., Camargo, M.E., Lafon, S., Degrave, W.M., Rangel-Aldao, R., Recombinant Trypanosoma cruzi antigens and Chagas' disease diagnosis: Analysis of a workshop (1991) FEMS Microbiol Immunol, 89, pp. 11-20
  • Umezawa, E.S., Bastos, S.F., Camargo, M.E., Yamauchi, L.M., Santos, M.R., Gonzalez, A., Zingales, B., Franco, D.S.J., Evaluation of recombinant antigens for serodiagnosis of Chagas' disease in South and Central America (1999) Journal of Clinical Microbiology, 37 (5), pp. 1554-1560
  • Gomes, Y.M., Lorena, V.M., Luquetti, A.O., Diagnosis of Chagas disease: What has been achieved? What remains to be done with regard to diagnosis and follow up studies? (2009) Mem Inst Oswaldo Cruz, 104 (SUPPL. I), pp. 115-121
  • Foti, L., Fonseca, B.D.P., Nascimento, L.D., Marques, C.D.F., Da Silva, E.D., Duarte, C.A., Probst, C.M., Krieger, M.A., Viability study of a multiplex diagnostic platform for Chagas disease (2009) Mem Inst Oswaldo Cruz, 104 (1 SUPPL.), pp. 136-141
  • Levin, M.J., Mesri, E., Benarous, R., Levitus, G., Schijman, A., Levy-Yeyati, P., Chiale, P.A., Segura, E.L., Identification of major Trypanosoma cruzi antigenic determinants in chronic Chagas' heart disease (1989) American Journal of Tropical Medicine and Hygiene, 41 (5), pp. 530-538
  • Tanowitz, H.B., Huang, H., Jelicks, L.A., Chandra, M., Loredo, M.L., Weiss, L.M., Factor, S.M., Yanagisawa, M., Role of endothelin 1 in the pathogenesis of chronic chagasic heart disease (2005) Infection and Immunity, 73 (4), pp. 2496-2503. , DOI 10.1128/IAI.73.4.2496-2503.2005
  • Talvani, A., Rocha, M.O.C., Barcelos, L.S., Gomes, Y.M., Ribeiro, A.L., Teixeira, M.M., Elevated concentrations of CCL2 and tumor necrosis factor-α in chagasic cardiomyopathy (2004) Clinical Infectious Diseases, 38 (7), pp. 943-950. , DOI 10.1086/381892
  • Wang, Y., Moreira Mda, C., Heringer-Walther, S., Ebermann, L., Schultheiss, H.P., Wessel, N., Siems, W.E., Walther, T., Plasma ACE2 activity is an independent prognostic marker in Chagas'disease and equally potent as BNP (2010) J Card Fail, 16, pp. 157-163
  • Mahler, E., Hoebeke, J., Levin, M.J., Structural and functional complexity of the humoral response against the Trypanosoma cruzi ribosomal P2β protein in patients with chronic Chagas' heart disease (2004) Clinical and Experimental Immunology, 136 (3), pp. 527-534. , DOI 10.1111/j.1365-2249.2004.02480.x
  • Elies, R., Ferrari, I., Wallukat, G., Lebesgue, D., Chiale, P., Elizari, M., Rosenbaum, M., Levin, M.J., Structural and Functional Analysis of the B Cell Epitopes Recognized by Anti-Receptor Autoantibodies in Patients with Chagas' Disease (1996) Journal of Immunology, 157 (9), pp. 4203-4211
  • Kaplan, D., Ferrari, I., Bergami, P.L., Mahler, E., Levitus, G., Chiale, P., Hoebeke, J., Levin, M.J., Antibodies to ribosomal P proteins of Trypanosoma cruzi in Chagas disease possess functional autoreactivity with heart tissue and differ from anti-P autoantibodies in lupus (1997) Proceedings of the National Academy of Sciences of the United States of America, 94 (19), pp. 10301-10306. , DOI 10.1073/pnas.94.19.10301

Citas:

---------- APA ----------
Longhi, S.A., Brandariz, S.B., Lafon, S.O., Niborski, L.L., Luquetti, A.O., Schijman, A.G., Levin, M.J.,..., Gómez, K.A. (2012) . Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms. American Journal of Tropical Medicine and Hygiene, 87(2), 267-271.
http://dx.doi.org/10.4269/ajtmh.2012.11-0533
---------- CHICAGO ----------
Longhi, S.A., Brandariz, S.B., Lafon, S.O., Niborski, L.L., Luquetti, A.O., Schijman, A.G., et al. "Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms" . American Journal of Tropical Medicine and Hygiene 87, no. 2 (2012) : 267-271.
http://dx.doi.org/10.4269/ajtmh.2012.11-0533
---------- MLA ----------
Longhi, S.A., Brandariz, S.B., Lafon, S.O., Niborski, L.L., Luquetti, A.O., Schijman, A.G., et al. "Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms" . American Journal of Tropical Medicine and Hygiene, vol. 87, no. 2, 2012, pp. 267-271.
http://dx.doi.org/10.4269/ajtmh.2012.11-0533
---------- VANCOUVER ----------
Longhi, S.A., Brandariz, S.B., Lafon, S.O., Niborski, L.L., Luquetti, A.O., Schijman, A.G., et al. Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms. Am. J. Trop. Med. Hyg. 2012;87(2):267-271.
http://dx.doi.org/10.4269/ajtmh.2012.11-0533