Artículo
Burgos, J.M.; Begher, S.B.; Freitas, J.M.; Bisio, M.; Duffy, T.; Altcheh, J.; Teijeiro, R.; Lopez Alcoba, H.; Deccarlini, F.; Freilij, H.; Levin, M.J.; Levalle, J.; Macedo, A.M.; Schijman, A.G. "Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS" (2005) American Journal of Tropical Medicine and Hygiene. 73(6):1016-1018
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Abstract:
Trypanosoma cruzi DNA was amplified from an intracranial biopsy and peripheral blood of an HIV patient with encephalitis; this episode was indicative of AIDS and congenital Chagas disease. The analysis of a microsatellite locus revealed a multiclonal parasite population at the brain lesion with a more complex minicircle signature than that profiled in blood using restriction fragment length polymorphism (RFLP)-PCR and low stringency single primer (LSSP) PCR. Interestingly, different sublineages of T. cruzi II were detected in blood and brain by means of spliced-leader and 24sα ribosomal-DNA amplifications. Quantitative-competitive PCR monitored the decrease of parasitic load during treatment and secondary prophylaxis with benznidazole. The synergy between parasiticidal plus antiretroviral treatments probably allowed the patient a longer survival than usually achieved in similar episodes. This is the first case report demonstrating a differential distribution of natural parasite populations and sublineages in Chagas disease reactivation, showing the proliferation of cerebral variants not detectable in peripheral blood. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene.
Registro:
| Documento: | Artículo |
| Título: | Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS |
| Autor: | Burgos, J.M.; Begher, S.B.; Freitas, J.M.; Bisio, M.; Duffy, T.; Altcheh, J.; Teijeiro, R.; Lopez Alcoba, H.; Deccarlini, F.; Freilij, H.; Levin, M.J.; Levalle, J.; Macedo, A.M.; Schijman, A.G. |
| Filiación: | Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética Biología (INGEBI-CONICET), Buenos Aires, Argentina Departamento de Fisiología y Biología Molecular y Cellular, FCEyN, UBA, Buenos Aires, Argentina Unidad 2 Clinica Médica e Infectología, Hospital Ignacio Pirovano, Gobierno de la Ciudad de Buenos Aires, Buenos Aires, Argentina Departamento de Bioquímica e Imunología, ICB, UFMG, Belo Horizonte, Brazil Laboratorio de Parasitología y Enfermedad de Chagas, Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina Vuelta de Obligado 2490, Buenos Aires 1428, Argentina |
| Palabras clave: | antiparasitic agent; antiretrovirus agent; benznidazole; protozoal DNA; ribosomal DNA 24salpha; ribosome DNA; unclassified drug; acquired immune deficiency syndrome; adult; article; brain biopsy; case report; Chagas disease; clone; diagnostic procedure; gene amplification; human; male; microorganism detection; molecular typing; nonhuman; polymerase chain reaction; quantitative analysis; restriction fragment length polymorphism; survival; Trypanosoma cruzi; virus encephalitis; Adult; AIDS-Related Opportunistic Infections; Animals; Chagas Disease; DNA, Kinetoplast; DNA, Protozoan; Encephalitis; Humans; Magnetic Resonance Imaging; Male; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Trypanosoma cruzi; Variation (Genetics); Trypanosoma cruzi |
| Año: | 2005 |
| Volumen: | 73 |
| Número: | 6 |
| Página de inicio: | 1016 |
| Página de fin: | 1018 |
| Título revista: | American Journal of Tropical Medicine and Hygiene |
| Título revista abreviado: | Am. J. Trop. Med. Hyg. |
| ISSN: | 00029637 |
| CODEN: | AJTHA |
| CAS: | benznidazole, 22994-85-0; DNA, Kinetoplast; DNA, Protozoan |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v73_n6_p1016_Burgos |
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- Vago, A.R., Macedo, A.M., Oliveira, R.P., Andrade, L.O., Chiari, E., Galvao, L.M., Reis, D., Pena, S.D., Kinetoplast DNA signatures of Trypanosoma cruzi strains obtained directly from infected tissues (1996) Am J Pathol, 149, pp. 2153-2159
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- Schijman, A.G., Vigliano, C., Burgos, J., Favaloro, R., Perrone, S., Laguens, R., Levin, M.J., Early diagnosis of recurrence of Trypanosoma cruzi infection by polymerase chain reaction after heart transplantation of a chronic Chagas' heart disease patient (2000) J Heart Lung Transplant, 19, pp. 1114-1117
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Citas:
---------- APA ----------
Burgos, J.M., Begher, S.B., Freitas, J.M., Bisio, M., Duffy, T., Altcheh, J., Teijeiro, R.,..., Schijman, A.G. (2005) . Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS. American Journal of Tropical Medicine and Hygiene, 73(6), 1016-1018.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v73_n6_p1016_Burgos [ ]
---------- CHICAGO ----------
Burgos, J.M., Begher, S.B., Freitas, J.M., Bisio, M., Duffy, T., Altcheh, J., et al. "Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS" . American Journal of Tropical Medicine and Hygiene 73, no. 6 (2005) : 1016-1018.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v73_n6_p1016_Burgos [ ]
---------- MLA ----------
Burgos, J.M., Begher, S.B., Freitas, J.M., Bisio, M., Duffy, T., Altcheh, J., et al. "Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS" . American Journal of Tropical Medicine and Hygiene, vol. 73, no. 6, 2005, pp. 1016-1018.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v73_n6_p1016_Burgos [ ]
---------- VANCOUVER ----------
Burgos, J.M., Begher, S.B., Freitas, J.M., Bisio, M., Duffy, T., Altcheh, J., et al. Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS. Am. J. Trop. Med. Hyg. 2005;73(6):1016-1018.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v73_n6_p1016_Burgos [ ]
