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It is still unclear to what extent myocarditis-associated, chronic Chagas' heart disease is due to persisting Trypanosoma cruzi. In the present study, we have analyzed tissue samples from the hearts of three patients with this disease. In situ hybridization provided little evidence for the presence of intact T. cruzi, even at sites of strong inflammation. Nevertheless, micromanipulation techniques detected remnants of both T. cruzi kinetoplast DNA and nuclear DNA. Trypanosoma cruzi DNA was also detected in single macrophages dissected directly from frozen heart tissue sections. Thus, this analysis demonstrates that T. cruzi kinetoplast DNA and nuclear DNA are widely dispersed in the heart tissue, although in low amounts. Since we rarely detected intact T. cruzi parasites during the chronic phase of Chagas' heart disease, we can exclude heart tissue as a major parasite reservoir.


Documento: Artículo
Título:Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease
Autor:Elias, F.E.; Vigliano, C.A.; Laguens, R.P.; Levin, M.J.; Berek, C.
Filiación:Genethor GmbH, Robert-Rössle-Strasse 10, D-13125 Berlin, Germany
Departamento de Anatomia Patologica, Inst. de Cardiol. y Cir. Cardiovasc., Fundación Favaloro, Solís 453, 1428 Buenos Aires, Argentina
Inst. Invest. Ing. Genet./Biol. Mol., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Deutsches Rheuma-ForschungsZentrum, Schumannstrasse 20/21, 10117 Berlin, Germany
Palabras clave:cell nucleus DNA; kinetoplast DNA; protozoal DNA; primer DNA; protozoal DNA; adult; animal experiment; animal model; animal tissue; article; case report; Chagas disease; controlled study; female; heart; human; human tissue; in situ hybridization; inflammation; macrophage; male; mouse; myocarditis; nonhuman; persistent infection; tissue section; Trypanosoma cruzi; zoonosis; animal; Bagg albino mouse; Chagas disease; chronic disease; genetics; heart muscle; isolation and purification; middle aged; molecular genetics; myocarditis; nucleotide sequence; parasitology; pathology; polymerase chain reaction; Animalia; Trypanosoma; Trypanosoma cruzi; Animals; Base Sequence; Chagas Cardiomyopathy; Chronic Disease; DNA Primers; DNA, Protozoan; Female; Humans; Male; Mice; Mice, Inbred BALB C; Middle Aged; Molecular Sequence Data; Myocarditis; Myocardium; Polymerase Chain Reaction; Trypanosoma cruzi
Página de inicio:242
Página de fin:247
Título revista:American Journal of Tropical Medicine and Hygiene
Título revista abreviado:Am. J. Trop. Med. Hyg.
CAS:DNA Primers; DNA, Protozoan


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---------- APA ----------
Elias, F.E., Vigliano, C.A., Laguens, R.P., Levin, M.J. & Berek, C. (2003) . Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease. American Journal of Tropical Medicine and Hygiene, 68(2), 242-247.
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---------- CHICAGO ----------
Elias, F.E., Vigliano, C.A., Laguens, R.P., Levin, M.J., Berek, C. "Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease" . American Journal of Tropical Medicine and Hygiene 68, no. 2 (2003) : 242-247.
Recuperado de [ ]
---------- MLA ----------
Elias, F.E., Vigliano, C.A., Laguens, R.P., Levin, M.J., Berek, C. "Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease" . American Journal of Tropical Medicine and Hygiene, vol. 68, no. 2, 2003, pp. 242-247.
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---------- VANCOUVER ----------
Elias, F.E., Vigliano, C.A., Laguens, R.P., Levin, M.J., Berek, C. Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease. Am. J. Trop. Med. Hyg. 2003;68(2):242-247.
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