Artículo

Debes, M.A.; Arias, M.E.; Grellet-Bournonville, C.F.; Wulff, A.F.; Martínez-Zamora, M.G.; Castagnaro, A.P.; Díaz-Ricci, J.C. "White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression" (2011) American Journal of Botany. 98(12):2077-2083
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Abstract:

Premise of the study: Duchesnea indica is a wild strawberry-like species that has red fruits. In a recent survey in the highlands of Tucum á n (Argentina), a plant of D. indica with white fruits was discovered. The aim of this study was to investigate whether the white-fruited character was due to a phenotypic or genotypic change. The stability and heritability of the character and the expression of genes involved in anthocyanins synthesis were studied and compared with red-fruited genotypes. This study contributes to understanding the molecular basis of some factors involved in fruit pigmentation, a horticulturally and taxonomically important trait. Methods: Stability and heritability of the white-fruited character were evaluated in plants obtained by asexual propagation or by sexual crosses between the white- and red-fruited genotypes. Asexual multiplications were carried out by stolon rooting and sexual multiplications by germination of achenes obtained from crosses. The expression level of the genes involved in the synthesis and regulation of the anthocyanins pathway (CHS, F3H, DFR, ANS, and MYB10) were evaluated by RT-PCR using specific primers. Key results: Plants with the white-fruited character always yielded white-fruited progeny when propagated asexually, whereas in sexually propagated plants fruit color depended on the mother. Red-fruited mothers yielded red-fruited progeny, and whitefruited mothers yielded fruits ranging from dark pink to white. Molecular analysis suggested that the white-fruited character was due to the low expression of the ANS gene. Conclusions: Results obtained indicate that the white-fruited character was stable. Mother progenitors exert a strong influence on the expression of the white-fruited character. The white-fruited phenotype is due to the impairment or downregulation of the ANS gene. © 2011 Botanical Society of America.

Registro:

Documento: Artículo
Título:White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression
Autor:Debes, M.A.; Arias, M.E.; Grellet-Bournonville, C.F.; Wulff, A.F.; Martínez-Zamora, M.G.; Castagnaro, A.P.; Díaz-Ricci, J.C.
Filiación:Facultad de Ciencias Naturales, Universidad Nacional de Tucumán (UNT), Tucumán, Argentina
Instituto Superior de Investigaciones Biológicas (INSIBIO, UNT-CONICET), Tucumán, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Sección Biotecnología, Estación Experimental Agroindustrial Obispo Colombres (EEAOC), Unidad Asociada a INSIBIO, Tucumán, Argentina
Palabras clave:ANS; Anthocyanin; Gene expression; Intra-specific cross; MYB10; Rosaceae; White-fruited Duchesnea indica; anthocyanin; vegetable protein; color; fruit; gene expression; genotype; germination; heritability; horticulture; life history trait; molecular analysis; phenotype; pigment; taxonomy; wild population; article; biosynthesis; cross breeding; flower; fruit; gene expression regulation; genetics; genotype; histology; metabolism; pigmentation; plant gene; plant leaf; Rosaceae; species difference; Anthocyanins; Crosses, Genetic; Flowers; Fruit; Gene Expression Regulation, Plant; Genes, Plant; Genotype; Pigmentation; Plant Leaves; Plant Proteins; Rosaceae; Species Specificity; Argentina; Tucuman; Duchesnea indica; Fragaria x ananassa; Rosaceae
Año:2011
Volumen:98
Número:12
Página de inicio:2077
Página de fin:2083
DOI: http://dx.doi.org/10.3732/ajb.1000373
Título revista:American Journal of Botany
Título revista abreviado:Am. J. Bot.
ISSN:00029122
CODEN:AJBOA
CAS:Anthocyanins; Plant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029122_v98_n12_p2077_Debes

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Citas:

---------- APA ----------
Debes, M.A., Arias, M.E., Grellet-Bournonville, C.F., Wulff, A.F., Martínez-Zamora, M.G., Castagnaro, A.P. & Díaz-Ricci, J.C. (2011) . White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression. American Journal of Botany, 98(12), 2077-2083.
http://dx.doi.org/10.3732/ajb.1000373
---------- CHICAGO ----------
Debes, M.A., Arias, M.E., Grellet-Bournonville, C.F., Wulff, A.F., Martínez-Zamora, M.G., Castagnaro, A.P., et al. "White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression" . American Journal of Botany 98, no. 12 (2011) : 2077-2083.
http://dx.doi.org/10.3732/ajb.1000373
---------- MLA ----------
Debes, M.A., Arias, M.E., Grellet-Bournonville, C.F., Wulff, A.F., Martínez-Zamora, M.G., Castagnaro, A.P., et al. "White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression" . American Journal of Botany, vol. 98, no. 12, 2011, pp. 2077-2083.
http://dx.doi.org/10.3732/ajb.1000373
---------- VANCOUVER ----------
Debes, M.A., Arias, M.E., Grellet-Bournonville, C.F., Wulff, A.F., Martínez-Zamora, M.G., Castagnaro, A.P., et al. White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression. Am. J. Bot. 2011;98(12):2077-2083.
http://dx.doi.org/10.3732/ajb.1000373