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Yushchenko, D.A.; Fauerbach, J.A.; Thirunavukkuarasu, S.; Jares-Erijman, E.A.; Jovin, T.M. "Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation" (2010) Journal of the American Chemical Society. 132(23):7860-7861
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Abstract:

We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of α-synuclein aggregation, a process associated with Parkinsons disease. © 2010 American Chemical Society.

Registro:

Documento: Artículo
Título:Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
Autor:Yushchenko, D.A.; Fauerbach, J.A.; Thirunavukkuarasu, S.; Jares-Erijman, E.A.; Jovin, T.M.
Filiación:Laboratory of Cellular Dynamics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:Continuous monitoring; Dual emissions; Fluorescent probes; Intermediate stage; Parkinsons disease; Protein aggregation; Ratiometric; Sensor molecules; Synuclein; Probes; Fluorescence; acetylcysteine; alanine; alpha synuclein; amyloid; chromone derivative; cysteine; maleimide; thiol; article; fluorescence spectroscopy; molecular sensor; protein aggregation; proton transport; synthesis; alpha-Synuclein; Fluorescent Dyes; Maleimides; Models, Molecular; Protein Multimerization; Protein Structure, Quaternary; Spectrometry, Fluorescence; Sulfhydryl Compounds; Time Factors
Año:2010
Volumen:132
Número:23
Página de inicio:7860
Página de fin:7861
DOI: http://dx.doi.org/10.1021/ja102838n
Título revista:Journal of the American Chemical Society
Título revista abreviado:J. Am. Chem. Soc.
ISSN:00027863
CODEN:JACSA
CAS:acetylcysteine, 616-91-1; alanine, 56-41-7, 6898-94-8; alpha synuclein, 154040-18-3; amyloid, 11061-24-8; cysteine, 4371-52-2, 52-89-1, 52-90-4; maleimide, 541-59-3; Fluorescent Dyes; Maleimides; Sulfhydryl Compounds; alpha-Synuclein
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v132_n23_p7860_Yushchenko

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Citas:

---------- APA ----------
Yushchenko, D.A., Fauerbach, J.A., Thirunavukkuarasu, S., Jares-Erijman, E.A. & Jovin, T.M. (2010) . Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation. Journal of the American Chemical Society, 132(23), 7860-7861.
http://dx.doi.org/10.1021/ja102838n
---------- CHICAGO ----------
Yushchenko, D.A., Fauerbach, J.A., Thirunavukkuarasu, S., Jares-Erijman, E.A., Jovin, T.M. "Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation" . Journal of the American Chemical Society 132, no. 23 (2010) : 7860-7861.
http://dx.doi.org/10.1021/ja102838n
---------- MLA ----------
Yushchenko, D.A., Fauerbach, J.A., Thirunavukkuarasu, S., Jares-Erijman, E.A., Jovin, T.M. "Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation" . Journal of the American Chemical Society, vol. 132, no. 23, 2010, pp. 7860-7861.
http://dx.doi.org/10.1021/ja102838n
---------- VANCOUVER ----------
Yushchenko, D.A., Fauerbach, J.A., Thirunavukkuarasu, S., Jares-Erijman, E.A., Jovin, T.M. Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation. J. Am. Chem. Soc. 2010;132(23):7860-7861.
http://dx.doi.org/10.1021/ja102838n