Abstract:
We report a fully integrated core-shell nanoparticle system responsive to glucose. The system is comprised of self-assembled glucose oxidase and an osmium molecular wire on core-shell Au nanoparticles. Characterization of the functional nanoparticles by spectroscopy, quartz crystal microbalance and electrochemical techniques has shown that the catalytically active shell has a structure as designed and all components are active in the self-assembled multilayer shell. Furthermore, amperometric reagentless detection of glucose and contactless photonic biosensing by the Os(II) resonant Raman signal have been demonstrated. The enzymatic reduction of FAD by glucose and further reduction of the Raman silent Os(III) by FADH2 yields a characteristic enzyme-substrate calibration curve in the millimolar range. Furthermore, coupling of electronic resonant Raman of the osmium complex with the SERS amplification by Au NPs plasmon resonance has been demonstrated which leads to an extra enhancement of the biosensor signal. We present a proof of concept extending the work done with planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected by SERS-like methods. © 2008 American Chemical Society.
Registro:
Documento: |
Artículo
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Título: | Wired-enzyme core-shell Au nanoparticle biosensor |
Autor: | Scodeller, P.; Flexer, V.; Szamocki, R.; Calvo, E.J.; Tognalli, N.; Troiani, H.; Fainstein, A. |
Filiación: | INQUIMAE, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina Centro Atomico Bariloche and Instituto Balseiro, Comision Nacional de Energia Atomica, 8400 S. C. de Bariloche, Argentina
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Palabras clave: | Biosensors; Computer operating systems; Crystal structure; Food additives; Glucose; Glucose oxidase; Glucose sensors; Nanoparticles; Nanostructured materials; Nanostructures; Osmium; Oxide minerals; Quartz; Quartz crystal microbalances; Reduction; Shells (structures); Amperometric; Au nano-particles; Bio sensor; Bio-sensing; Contactless; Core shells; Electrochemical techniques; Enzymatic reduction; Fully integrated; Functional nanoparticles; Glucose oxidases; Molecular wires; Photonic; Quartz crystal micro-balance; Reagent-less; Resonant Raman; Self-assembled; Self-assembled multilayer; Gold; flavine adenine nucleotide; flavine mononucleotide reductase; glucose; glucose oxidase; gold; nanoparticle; nanowire; osmium; silicon dioxide; amperometry; article; biosensor; calibration; catalysis; electrochemical analysis; enzyme substrate complex; oxidation; Raman spectrometry; reduction; signal transduction; spectroscopy; surface property; synthesis; Biosensing Techniques; Calibration; Electrochemical Techniques; Flavin-Adenine Dinucleotide; Glucose; Glucose Oxidase; Gold; Metal Nanoparticles; Microscopy, Atomic Force; Microscopy, Electron, Transmission; Nanowires; Osmium; Spectrophotometry, Ultraviolet; Spectrum Analysis, Raman |
Año: | 2008
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Volumen: | 130
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Número: | 38
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Página de inicio: | 12690
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Página de fin: | 12697
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DOI: |
http://dx.doi.org/10.1021/ja802318f |
Título revista: | Journal of the American Chemical Society
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Título revista abreviado: | J. Am. Chem. Soc.
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ISSN: | 00027863
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CODEN: | JACSA
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CAS: | flavine adenine nucleotide, 146-14-5; flavine mononucleotide reductase, 39346-42-4, 64295-83-6; glucose oxidase, 9001-37-0; glucose, 50-99-7, 84778-64-3; gold, 7440-57-5; osmium, 7440-04-2; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; 1,5-dihydro-FAD, 1910-41-4; Flavin-Adenine Dinucleotide, 146-14-5; Glucose Oxidase, EC 1.1.3.4; Glucose, 50-99-7; Gold, 7440-57-5; Osmium, 7440-04-2
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v130_n38_p12690_Scodeller |
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Citas:
---------- APA ----------
Scodeller, P., Flexer, V., Szamocki, R., Calvo, E.J., Tognalli, N., Troiani, H. & Fainstein, A.
(2008)
. Wired-enzyme core-shell Au nanoparticle biosensor. Journal of the American Chemical Society, 130(38), 12690-12697.
http://dx.doi.org/10.1021/ja802318f---------- CHICAGO ----------
Scodeller, P., Flexer, V., Szamocki, R., Calvo, E.J., Tognalli, N., Troiani, H., et al.
"Wired-enzyme core-shell Au nanoparticle biosensor"
. Journal of the American Chemical Society 130, no. 38
(2008) : 12690-12697.
http://dx.doi.org/10.1021/ja802318f---------- MLA ----------
Scodeller, P., Flexer, V., Szamocki, R., Calvo, E.J., Tognalli, N., Troiani, H., et al.
"Wired-enzyme core-shell Au nanoparticle biosensor"
. Journal of the American Chemical Society, vol. 130, no. 38, 2008, pp. 12690-12697.
http://dx.doi.org/10.1021/ja802318f---------- VANCOUVER ----------
Scodeller, P., Flexer, V., Szamocki, R., Calvo, E.J., Tognalli, N., Troiani, H., et al. Wired-enzyme core-shell Au nanoparticle biosensor. J. Am. Chem. Soc. 2008;130(38):12690-12697.
http://dx.doi.org/10.1021/ja802318f