Abstract:
The replacement of the axial methionine ligand in a native CuA protein rendered a series of stable mutants with spectroscopic features of a mixed valence center. The mutations resulted in minor perturbations of the electronic structure of this site but led to significant changes in the redox potential. This suggests that the axial ligand plays a role in tuning the redox potential of CuA, resembling the behavior observed in blue copper sites. Copyright © 2007 American Chemical Society.
Registro:
Documento: |
Artículo
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Título: | The met axial ligand determines the redox potential in CuA sites |
Autor: | Ledesma, G.N.; Murgida, D.H.; Hoang, K.L.; Wackerbarth, H.; Ulstrup, J.; Costa-Filho, A.J.; Vila, A.J. |
Filiación: | Instituto de Biología Molecular Y Celular de Rosario-CONICET, Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina Max Volmer Laboratory of Biophysical Chemistry, Institute of Chemistry, Technical University of Berlin, Strasse des 17. Juni, D-10623, Berlin, Germany INQUIMAE-CONICET, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina Department of Chemistry and NanoDTU, Technical University of Denmark, DK-2800 Kgs, Lyngby, Denmark Instituto de Física de São Carlos, Universidade de São Paulo, C.P. 369, CEP 13560-950, São Carlos, SP, Brazil
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Palabras clave: | copper; ligand; met axial ligand; unclassified drug; article; bacillus denitrificans fluorescens; electron spin resonance; electron transport; hydrophobicity; oxidation reduction reaction; Pseudomonas; Rhodobacter sphaeroides; Azurin; Copper; Electron Spin Resonance Spectroscopy; Ligands; Models, Molecular; Oxidation-Reduction; Spectrophotometry, Ultraviolet |
Año: | 2007
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Volumen: | 129
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Número: | 39
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Página de inicio: | 11884
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Página de fin: | 11885
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DOI: |
http://dx.doi.org/10.1021/ja0731221 |
Título revista: | Journal of the American Chemical Society
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Título revista abreviado: | J. Am. Chem. Soc.
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ISSN: | 00027863
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CODEN: | JACSA
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CAS: | copper, 15158-11-9, 7440-50-8; Azurin, 12284-43-4; Copper, 7440-50-8; Ligands
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v129_n39_p11884_Ledesma |
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Citas:
---------- APA ----------
Ledesma, G.N., Murgida, D.H., Hoang, K.L., Wackerbarth, H., Ulstrup, J., Costa-Filho, A.J. & Vila, A.J.
(2007)
. The met axial ligand determines the redox potential in CuA sites. Journal of the American Chemical Society, 129(39), 11884-11885.
http://dx.doi.org/10.1021/ja0731221---------- CHICAGO ----------
Ledesma, G.N., Murgida, D.H., Hoang, K.L., Wackerbarth, H., Ulstrup, J., Costa-Filho, A.J., et al.
"The met axial ligand determines the redox potential in CuA sites"
. Journal of the American Chemical Society 129, no. 39
(2007) : 11884-11885.
http://dx.doi.org/10.1021/ja0731221---------- MLA ----------
Ledesma, G.N., Murgida, D.H., Hoang, K.L., Wackerbarth, H., Ulstrup, J., Costa-Filho, A.J., et al.
"The met axial ligand determines the redox potential in CuA sites"
. Journal of the American Chemical Society, vol. 129, no. 39, 2007, pp. 11884-11885.
http://dx.doi.org/10.1021/ja0731221---------- VANCOUVER ----------
Ledesma, G.N., Murgida, D.H., Hoang, K.L., Wackerbarth, H., Ulstrup, J., Costa-Filho, A.J., et al. The met axial ligand determines the redox potential in CuA sites. J. Am. Chem. Soc. 2007;129(39):11884-11885.
http://dx.doi.org/10.1021/ja0731221