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Abstract:

We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 Å) than any previously obtained with biarsenical dyes. Copyright © 2006 American Chemical Society.

Registro:

Documento: Artículo
Título:Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins
Autor:Spagnuolo, C.C.; Vermeij, R.J.; Jares-Erijman, E.A.
Filiación:Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina
Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany
Biophysical Engineering Group, Faculty of Science and Technology, University of Twente, 7500 AE Enschede, Netherlands
Palabras clave:arsenic derivative; biotin; cysteine derivative; dye; fluorescein; absorption spectroscopy; anisotropy; article; chemical structure; complex formation; fluorescence; fluorescence resonance energy transfer; fluorescence spectroscopy; fluorination; irradiation; pH; photochemistry; Arsenicals; Cysteine; Drug Stability; Fluoresceins; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Hydrocarbons, Fluorinated; Hydrogen-Ion Concentration; Oligopeptides; Photochemistry
Año:2006
Volumen:128
Número:37
Página de inicio:12040
Página de fin:12041
DOI: http://dx.doi.org/10.1021/ja063212q
Título revista:Journal of the American Chemical Society
Título revista abreviado:J. Am. Chem. Soc.
ISSN:00027863
CODEN:JACSA
CAS:biotin, 58-85-5; fluorescein, 2321-07-5, 91316-42-6; Arsenicals; Cysteine, 52-90-4; Fluoresceins; Hydrocarbons, Fluorinated; Oligopeptides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v128_n37_p12040_Spagnuolo

Referencias:

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Citas:

---------- APA ----------
Spagnuolo, C.C., Vermeij, R.J. & Jares-Erijman, E.A. (2006) . Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins. Journal of the American Chemical Society, 128(37), 12040-12041.
http://dx.doi.org/10.1021/ja063212q
---------- CHICAGO ----------
Spagnuolo, C.C., Vermeij, R.J., Jares-Erijman, E.A. "Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins" . Journal of the American Chemical Society 128, no. 37 (2006) : 12040-12041.
http://dx.doi.org/10.1021/ja063212q
---------- MLA ----------
Spagnuolo, C.C., Vermeij, R.J., Jares-Erijman, E.A. "Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins" . Journal of the American Chemical Society, vol. 128, no. 37, 2006, pp. 12040-12041.
http://dx.doi.org/10.1021/ja063212q
---------- VANCOUVER ----------
Spagnuolo, C.C., Vermeij, R.J., Jares-Erijman, E.A. Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins. J. Am. Chem. Soc. 2006;128(37):12040-12041.
http://dx.doi.org/10.1021/ja063212q