Artículo
Calvo, E.J.; Danilowicz, C.; Wolosiuk, A. "Molecular "wiring" enzymes in organized nanostructures" (2002) Journal of the American Chemical Society. 124(11):2452-2453
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Abstract:
We report on the "molecular wiring" efficiency of glucose oxidase in organized self-assembled nanostructures comprised of enzyme layers alternating with layers of an osmium-derivatized poly(allylamine) cationic polyelectrolyte, acting as redox relays. Varying the relative position of the active enzyme layer in nanostructures alternating active enzyme and inactive apoenzyme we have demonstrated that the specific rate of bimolecular FADH2 oxidation ("wiring efficiency") is limited by the diffusion-like electron hopping mechanism in the multilayers. Copyright © 2002 American Chemical Society.
Registro:
| Documento: | Artículo |
| Título: | Molecular "wiring" enzymes in organized nanostructures |
| Autor: | Calvo, E.J.; Danilowicz, C.; Wolosiuk, A. |
| Filiación: | INQUIMAE-Departamento de Quimica Inorganica, Analitica y Quimica Fisca, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, AR-1428 Buenos Aires, Argentina |
| Palabras clave: | apoenzyme; avidin; biotin; enzyme; glucose; glucose oxidase; osmium; polyelectrolyte; polymer; single stranded DNA; adsorption; amperometry; antigen antibody reaction; article; biosensor; cyclic potentiometry; diffusion; diffusion coefficient; DNA hybridization; electricity; electrochemical analysis; electron transport; electronics; enzyme immobilization; enzyme structure; glucose oxidation; hydrogel; isoelectric point; Michaelis constant; molecular recognition; oxidation; pH; steady state; Electrochemistry; Glucose; Glucose Oxidase; Nanotechnology; Organometallic Compounds; Osmium; Oxidation-Reduction; Pyridines |
| Año: | 2002 |
| Volumen: | 124 |
| Número: | 11 |
| Página de inicio: | 2452 |
| Página de fin: | 2453 |
| DOI: | http://dx.doi.org/10.1021/ja017802u |
| Título revista: | Journal of the American Chemical Society |
| Título revista abreviado: | J. Am. Chem. Soc. |
| ISSN: | 00027863 |
| CODEN: | JACSA |
| CAS: | biotin, 58-85-5; glucose, 50-99-7, 84778-64-3; glucose oxidase, 9001-37-0; osmium, 7440-04-2; Glucose, 50-99-7; Glucose Oxidase, 1.1.3.4; Organometallic Compounds; Osmium, 7440-04-2; Pyridines |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v124_n11_p2452_Calvo |
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Citas:
---------- APA ----------
Calvo, E.J., Danilowicz, C. & Wolosiuk, A. (2002) . Molecular "wiring" enzymes in organized nanostructures. Journal of the American Chemical Society, 124(11), 2452-2453.http://dx.doi.org/10.1021/ja017802u
---------- CHICAGO ----------
Calvo, E.J., Danilowicz, C., Wolosiuk, A. "Molecular "wiring" enzymes in organized nanostructures" . Journal of the American Chemical Society 124, no. 11 (2002) : 2452-2453.http://dx.doi.org/10.1021/ja017802u
---------- MLA ----------
Calvo, E.J., Danilowicz, C., Wolosiuk, A. "Molecular "wiring" enzymes in organized nanostructures" . Journal of the American Chemical Society, vol. 124, no. 11, 2002, pp. 2452-2453.http://dx.doi.org/10.1021/ja017802u
---------- VANCOUVER ----------
Calvo, E.J., Danilowicz, C., Wolosiuk, A. Molecular "wiring" enzymes in organized nanostructures. J. Am. Chem. Soc. 2002;124(11):2452-2453.http://dx.doi.org/10.1021/ja017802u
