Artículo
Schijman, A.G.; Lauricella, M.A.; Marcet, P.L.; Duffy, T.; Cardinal, M.V.; Bisio, M.; Levin, M.J.; Kitron, U.; Gürtler, R.E. "Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina" (2006) Acta Tropica. 99(1):50-54
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Abstract:
Flagellates indistinguishable from Trypanosoma cruzi were detected by microscopy in faecal samples of 2/110 Triatoma guasayana and 2/283 Triatoma garciabesi captured in a rural area of northwestern Argentina. Inoculation of faecal homogenates to mice followed by xenodiagnosis, haemoculture, histopathology and culture from cardiac homogenates, and PCR based on T. cruzi minicircle and nuclear sequences failed to detect T. cruzi infection, pointing to another trypanosomatidean. A PCR strategy targeted to the D7 domain of 24sα ribosomal DNA genes amplified a 250 bp sequence from one T. guasayana and one T. garciabesi faecal lysate. Sequence analysis revealed 100% identity with 24sα rDNA amplicons from Blastocrithidia triatomae obtained from faeces of reared Triatoma infestans bugs. Phylogenetic analysis clustered this sequence with C. fasciculata and L. major, separated from the Trypanosoma branch (bootstrap: 968/1000), in concordance with a Neighbour-joining dendrogram based on 18s rDNA sequences. This PCR procedure provides a rapid sensitive tool for differential diagnosis of morphologically similar trypanosomatids in field surveys of Chagas disease vectors and laboratory-reared triatomines used for xenodiagnosis. © 2006 Elsevier B.V. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina |
| Autor: | Schijman, A.G.; Lauricella, M.A.; Marcet, P.L.; Duffy, T.; Cardinal, M.V.; Bisio, M.; Levin, M.J.; Kitron, U.; Gürtler, R.E. |
| Filiación: | Laboratorio de Biología Molecular de la Enfermedad de Chagas (LabMeCh), Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Argentina Instituto Nacional de Parasitología Dr. Mario Fatala Chabén, Buenos Aires, Argentina College of Veterinary Medicine, University of Illinois, Urbana-Champaign, IL, United States |
| Idioma: | Inglés |
| Palabras clave: | 24sα ribosomal RNA genes; Blastocrithidia triatomae; Chagas disease; Triatoma garciabesi; Triatoma guasayana; Trypanosoma cruzi; DNA 18S; ribosome DNA; fecal pellet; flagellate; histopathology; inoculation; insect; microscopy; phylogenetics; polymerase chain reaction; RNA; rodent; rural area; amplicon; animal experiment; animal tissue; Argentina; article; blastocrithidia triatomae; blood culture; Crithidia fasciculata; disease carrier; DNA sequence; feces; flagellate; gene amplification; gene sequence; heart; histopathology; homogenate; inoculation; Leishmania major; microorganism detection; microscopy; morphology; mouse; neighbor joining method; nonhuman; nucleotide sequence; phylogenetic tree; phylogeny; polymerase chain reaction; protozoal infection; RNA gene; rural area; sample; sequence analysis; tissue culture; Triatoma; triatoma garciabesi; triatoma guasayana; Triatoma infestans; Triatominae; Trypanosoma; Trypanosoma cruzi; trypanosomiasis; xenodiagnosis; Animals; Argentina; Base Sequence; DNA, Kinetoplast; Feces; Insect Vectors; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction; RNA, Ribosomal; Rural Population; Sequence Alignment; Sequence Analysis, DNA; Triatoma; Trypanosoma cruzi; Trypanosomatina; Xenodiagnosis; Argentina; South America; Blastocrithidia triatomiae; Mastigophora (flagellates); Triatoma garciabesi; Triatoma guasayana; Triatoma infestans; Trypanosoma; Trypanosoma cruzi; Trypanosomatidae |
| Año: | 2006 |
| Volumen: | 99 |
| Número: | 1 |
| Página de inicio: | 50 |
| Página de fin: | 54 |
| DOI: | http://dx.doi.org/10.1016/j.actatropica.2006.06.010 |
| Título revista: | Acta Tropica |
| Título revista abreviado: | Acta Trop. |
| ISSN: | 0001706X |
| CODEN: | ACTRA |
| CAS: | DNA, Kinetoplast; RNA, Ribosomal |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v99_n1_p50_Schijman |
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Citas:
---------- APA ----------
Schijman, A.G., Lauricella, M.A., Marcet, P.L., Duffy, T., Cardinal, M.V., Bisio, M., Levin, M.J.,..., Gürtler, R.E. (2006) . Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina. Acta Tropica, 99(1), 50-54.http://dx.doi.org/10.1016/j.actatropica.2006.06.010
---------- CHICAGO ----------
Schijman, A.G., Lauricella, M.A., Marcet, P.L., Duffy, T., Cardinal, M.V., Bisio, M., et al. "Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina" . Acta Tropica 99, no. 1 (2006) : 50-54.http://dx.doi.org/10.1016/j.actatropica.2006.06.010
---------- MLA ----------
Schijman, A.G., Lauricella, M.A., Marcet, P.L., Duffy, T., Cardinal, M.V., Bisio, M., et al. "Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina" . Acta Tropica, vol. 99, no. 1, 2006, pp. 50-54.http://dx.doi.org/10.1016/j.actatropica.2006.06.010
---------- VANCOUVER ----------
Schijman, A.G., Lauricella, M.A., Marcet, P.L., Duffy, T., Cardinal, M.V., Bisio, M., et al. Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina. Acta Trop. 2006;99(1):50-54.http://dx.doi.org/10.1016/j.actatropica.2006.06.010
