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Abstract:

The larval (metacestode) stage of the tapeworm Echinococcus multilocularis causes alveolar echinococcosis (AE), a mainly hepatic disease characterized by continuous asexual proliferation of metacestodes by exogenous budding, resulting in the tumor-like, infiltrative growth of the parasite lesion. Current chemotherapeutical treatment of AE relies on the use of benzimidazoles, albendazole (ABZ) and mebendazole, but these drugs act parasitostatic rather than parasitocidal, and due to their low success rate they imply a lifelong application causing severe side effects. Thymol is one of the major components of the essential oils of Thymus and is a widely known anti-microbial agent. The aim of the present work was to compare the efficacy of albendazole (ABZ) and thymol separately or combined on E. multilocularis protoscoleces and metacestodes. For this purpose, microscopical examinations at different time points were carried out. Moreover the tegumentary enzyme gamma glutamyl transferase (GGT) was measured to quantify the damage in metacestodes. Even though treatments of in vitro cultured E. multilocularis protoscoleces or metacestodes with ABZ or/and thymol showed that the drugs have an adverse effect on parasite viability, the combination of the two compounds at the concentration of 10. μg/ml showed the maximum anti-parasitic effect. Three days postincubation the first effects of the treatment were detected on protoscoleces and a marked reduction in viability (33%) was registered at day 18. Incubation of E. multilocularis metacestodes in the presence of ABZ 10. μg/ml. +. thymol 10. μg/ml during 10 days resulted in dramatic alterations such as strongly irregular and fissured surface and markedly disrupted vesicles. Scanning electron microscopy showed that protoscoleces as well as the germinal layer of E. multilocularis metacestodes were dramatically damaged following ABZ or/and thymol treatment. Also an important increase of tegumentary enzyme GGT was registered after 72. h postincubation with both drugs. The data reported in this article demonstrate a clear in vitro effect of ABZ. +. thymol against E. multilocularis protoscoleces and metacestodes. © 2014 Elsevier B.V.

Registro:

Documento: Artículo
Título:Efficacy of albendazole in combination with thymol against Echinococcus multilocularis protoscoleces and metacestodes
Autor:María, A.C.; Celina, E.M.
Filiación:Laboratorio de Zoonosis Parasitarias, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Funes 3350, 7600 Mar del Plata, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Palabras clave:Albendazole; Chemotherapy; Echinococcus multilocularis; Metacestodes; Protoscoleces; Thymol; albendazole; gamma glutamyltransferase; thymol; albendazole; anthelmintic agent; thymol; albendazole; thymol; chemotherapy; disease treatment; drug; echinococcosis; enzyme; essential oil; flatworm; enzyme activity; larval development; vegetative propagation; antimicrobial activity; article; cestode life cycle stage; controlled study; cytotoxicity; drug efficacy; Echinococcus multilocularis; enzyme activity; enzyme assay; larval stage; membrane damage; metacestode; microscopy; morphology; nonhuman; parasite viability; protoscoleces; scanning electron microscopy; animal; drug combination; drug effects; Echinococcosis, Hepatic; Echinococcus multilocularis; inbred mouse strain; larva; mouse; Article; cestode life cycle stage; metacestode; protoscolece; Albendazole; Animals; Anthelmintics; Drug Therapy, Combination; Echinococcosis, Hepatic; Echinococcus multilocularis; Larva; Mice; Mice, Inbred Strains; Thymol; Echinococcus multilocularis
Año:2014
Volumen:140
Página de inicio:61
Página de fin:67
DOI: http://dx.doi.org/10.1016/j.actatropica.2014.08.007
Título revista:Acta Tropica
Título revista abreviado:Acta Trop.
ISSN:0001706X
CODEN:ACTRA
CAS:albendazole, 54965-21-8; gamma glutamyltransferase, 85876-02-4; thymol, 89-83-8; Albendazole; Anthelmintics; Thymol
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v140_n_p61_Maria

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Citas:

---------- APA ----------
María, A.C. & Celina, E.M. (2014) . Efficacy of albendazole in combination with thymol against Echinococcus multilocularis protoscoleces and metacestodes. Acta Tropica, 140, 61-67.
http://dx.doi.org/10.1016/j.actatropica.2014.08.007
---------- CHICAGO ----------
María, A.C., Celina, E.M. "Efficacy of albendazole in combination with thymol against Echinococcus multilocularis protoscoleces and metacestodes" . Acta Tropica 140 (2014) : 61-67.
http://dx.doi.org/10.1016/j.actatropica.2014.08.007
---------- MLA ----------
María, A.C., Celina, E.M. "Efficacy of albendazole in combination with thymol against Echinococcus multilocularis protoscoleces and metacestodes" . Acta Tropica, vol. 140, 2014, pp. 61-67.
http://dx.doi.org/10.1016/j.actatropica.2014.08.007
---------- VANCOUVER ----------
María, A.C., Celina, E.M. Efficacy of albendazole in combination with thymol against Echinococcus multilocularis protoscoleces and metacestodes. Acta Trop. 2014;140:61-67.
http://dx.doi.org/10.1016/j.actatropica.2014.08.007