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Abstract:

Human cystic echinococcosis is a zoonosis caused by the metacestode stage of the tapeworm Echinococcus granulosus. Although benzimidazole compounds such as albendazole and mebendazole have been the cornerstone of chemotherapy for the disease, there is often no complete recovery after treatment. Hence, in searching for novel treatment options, we examined the in vitro efficacies of 5-fluorouracil (5-FU) and paclitaxel (PTX) against E. granulosus germinal cells, protoscoleces and cysts. 5-FU or PTX inhibited the growth of E. granulosus cells in a time dependent manner. Although both treatments had a protoscolicidal effect, 5-FU had a considerably stronger effect than PTX. 5-FU produced a dose- and time-dependent effect, provoking the complete loss of viability after 24 days of incubation. Moreover, cysts did not develop following the inoculation of treated protoscoleces into mice. The loss of viability was slower in PTX treated protoscoleces, reaching to approximately 60% after 30 days. The results of the in vitro treatment with 5-FU and PTX were similar in secondary murine cysts. The employment of SEM and TEM allowed us to examine, at an ultrastructural level, the effects induced by 5-FU and PTX on E. granulosus germinal cells, protoscoleces and murine cysts. In conclusion, the data obtained clearly demonstrated that 5-FU and PTX at clinically achievable concentrations inhibit the survival of larval cells, protoscoleces and metacestodes. In vivo studies to test the antiparasitic activities of 5-FU and PTX are currently being undertaken on the murine model of cystic echinococcosis. © 2014 Elsevier B.V.

Registro:

Documento: Artículo
Título:In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells
Autor:Pensel, P.E.; Albani, C.; Gamboa, G.U.; Benoit, J.P.; Elissondo, M.C.
Filiación:Laboratorio de Zoonosis Parasitarias, Fac. Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CONICET, Mar del Plata, Argentina
Laboratorio de Farmacotecnia, Fac. Ciencias Químicas, Universidad Nacional de Córdoba, UNITEFA, Córdoba, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
INSERM U1066, MINT-Micro et Nanomédecines Biomimétiques, IBS-CHU Angers, 49933 Angers Cedex 9, France
Palabras clave:5-Fluorouracilo; Cyst echinococcosis; Echinococcus granulosus; Hydatid cysts; Paclitaxel; fluorouracil; paclitaxel; anticestodal agent; fluorouracil; paclitaxel; fluorouracil; paclitaxel; cyst; drug; echinococcosis; efficiency measurement; experiment; laboratory method; larva; parasite; survival; time dependent behavior; cell organelle; chemotherapy; disease treatment; incubation; inoculation; parasite infestation; animal cell; animal experiment; animal model; animal tissue; antiparasitic activity; article; cell growth; cell survival; cell type; cestode germinal cell; concentration response; controlled study; drug activity; drug efficacy; echinococcosis; Echinococcus granulosus; female; growth inhibition; in vitro study; metacestode; mouse; nonhuman; parasite viability; protoscolex; scanning electron microscopy; scolex; transmission electron microscopy; animal; dose response; drug effects; echinococcosis; Echinococcus granulosus; inbred mouse strain; larva; anthelmintic activity; Article; drug effect; echinococcosis; parasite survival; parasite virulence; Animals; Anticestodal Agents; Dose-Response Relationship, Drug; Echinococcosis; Echinococcus granulosus; Female; Fluorouracil; In Vitro Techniques; Larva; Mice; Mice, Inbred Strains; Paclitaxel; Echinococcus granulosus
Año:2014
Volumen:140
Página de inicio:1
Página de fin:9
DOI: http://dx.doi.org/10.1016/j.actatropica.2014.07.013
Título revista:Acta Tropica
Título revista abreviado:Acta Trop.
ISSN:0001706X
CODEN:ACTRA
CAS:fluorouracil, 51-21-8; paclitaxel, 33069-62-4; Anticestodal Agents; Fluorouracil; Paclitaxel
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v140_n_p1_Pensel

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Citas:

---------- APA ----------
Pensel, P.E., Albani, C., Gamboa, G.U., Benoit, J.P. & Elissondo, M.C. (2014) . In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells. Acta Tropica, 140, 1-9.
http://dx.doi.org/10.1016/j.actatropica.2014.07.013
---------- CHICAGO ----------
Pensel, P.E., Albani, C., Gamboa, G.U., Benoit, J.P., Elissondo, M.C. "In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells" . Acta Tropica 140 (2014) : 1-9.
http://dx.doi.org/10.1016/j.actatropica.2014.07.013
---------- MLA ----------
Pensel, P.E., Albani, C., Gamboa, G.U., Benoit, J.P., Elissondo, M.C. "In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells" . Acta Tropica, vol. 140, 2014, pp. 1-9.
http://dx.doi.org/10.1016/j.actatropica.2014.07.013
---------- VANCOUVER ----------
Pensel, P.E., Albani, C., Gamboa, G.U., Benoit, J.P., Elissondo, M.C. In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells. Acta Trop. 2014;140:1-9.
http://dx.doi.org/10.1016/j.actatropica.2014.07.013