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Abstract:

We assessed the diversity and distribution of Trypanosoma cruzi discrete typing units (DTU) in Triatoma infestans populations and its association with local vector-borne transmission levels at various geographic scales. At a local scale, we found high predominance (92.4%) of TcVI over TcV in 68 microscope-positive T. infestans collected in rural communities in Santiago del Estero province in northern Argentina. TcV was more often found in communities with higher house infestation prevalence compatible with active vector-borne transmission. Humans and dogs were the main bloodmeal sources of the TcV- and TcVI-infected bugs. At a broader scale, the greatest variation in DTU diversity was found within the Argentine Chaco (227 microscope-positive bugs), mainly related to differences in equitability between TcVI and TcV among study areas. At a country-wide level, a meta-analysis of published data revealed clear geographic variations in the distribution of DTUs across countries. A correspondence analysis showed that DTU distributions in domestic T. infestans were more similar within Argentina (dominated by TcVI) and within Bolivia (where TcI and TcV had similar relative frequencies), whereas large heterogeneity was found within Chile. DTU diversity was lower in the western Argentine Chaco region and Paraguay (D= 0.14-0.22) than in the eastern Argentine Chaco, Bolivia and Chile (D= 0.20-0.68). Simultaneous DTU identifications of T. cruzi-infected hosts and triatomines across areas differing in epidemiological status are needed to shed new light on the structure and dynamics of parasite transmission cycles. © 2014 Elsevier B.V.

Registro:

Documento: Artículo
Título:Geographic variation of Trypanosoma cruzi discrete typing units from Triatoma infestans at different spatial scales
Autor:Fernández, M.D.P.; Cecere, M.C.; Lanati, L.A.; Lauricella, M.A.; Schijman, A.G.; Gürtler, R.E.; Cardinal, M.V.
Filiación:Laboratory of Eco-Epidemiology, Department of Ecology, Genetics and Evolution, Universidad de Buenos Aires-IEGEBA (CONICET-UBA), Buenos Aires, Argentina
Instituto Nacional de Parasitología Dr. Mario Fatala Chaben, ANLIS, Buenos Aires, Argentina
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética, Biología Molecular Dr. Héctor Torres (INGEBI-CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina
Palabras clave:Discrete typing units; Diversity; Molecular epidemiology; Triatoma infestans; Trypanosoma cruzi; biodiversity; disease prevalence; disease transmission; epidemiology; geographical variation; molecular ecology; parasite; parasite infestation; protozoan; rural area; spatial analysis; animal cell; Argentina; article; Bolivia; chicken; Chile; controlled study; correspondence analysis; cross-sectional study; discrete typing unit; dog; geographical variation (species); goat; human; infestation; molecular genetics; nonhuman; Paraguay; parasite transmission; parasite vector; prevalence; rural population; species diversity; species identification; Triatoma infestans; Trypanosoma cruzi; animal; biodiversity; Chagas disease; disease carrier; isolation and purification; parasitology; Triatoma; Trypanosoma cruzi; Article; genotype; geographic distribution; molecular epidemiology; molecular typing; polymerase chain reaction; population distribution; population dynamics; population structure; seasonal variation; Argentina; Bolivia; Chaco [Argentina]; Chile; Paraguay; Santiago del Estero [Argentina]; Animals; Argentina; Biodiversity; Bolivia; Chagas Disease; Chile; Cross-Sectional Studies; Humans; Insect Vectors; Paraguay; Rural Population; Triatoma; Trypanosoma cruzi; Triatoma infestans; Trypanosoma cruzi
Año:2014
Volumen:140
Página de inicio:10
Página de fin:18
DOI: http://dx.doi.org/10.1016/j.actatropica.2014.07.014
Título revista:Acta Tropica
Título revista abreviado:Acta Trop.
ISSN:0001706X
CODEN:ACTRA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v140_n_p10_Fernandez

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Citas:

---------- APA ----------
Fernández, M.D.P., Cecere, M.C., Lanati, L.A., Lauricella, M.A., Schijman, A.G., Gürtler, R.E. & Cardinal, M.V. (2014) . Geographic variation of Trypanosoma cruzi discrete typing units from Triatoma infestans at different spatial scales. Acta Tropica, 140, 10-18.
http://dx.doi.org/10.1016/j.actatropica.2014.07.014
---------- CHICAGO ----------
Fernández, M.D.P., Cecere, M.C., Lanati, L.A., Lauricella, M.A., Schijman, A.G., Gürtler, R.E., et al. "Geographic variation of Trypanosoma cruzi discrete typing units from Triatoma infestans at different spatial scales" . Acta Tropica 140 (2014) : 10-18.
http://dx.doi.org/10.1016/j.actatropica.2014.07.014
---------- MLA ----------
Fernández, M.D.P., Cecere, M.C., Lanati, L.A., Lauricella, M.A., Schijman, A.G., Gürtler, R.E., et al. "Geographic variation of Trypanosoma cruzi discrete typing units from Triatoma infestans at different spatial scales" . Acta Tropica, vol. 140, 2014, pp. 10-18.
http://dx.doi.org/10.1016/j.actatropica.2014.07.014
---------- VANCOUVER ----------
Fernández, M.D.P., Cecere, M.C., Lanati, L.A., Lauricella, M.A., Schijman, A.G., Gürtler, R.E., et al. Geographic variation of Trypanosoma cruzi discrete typing units from Triatoma infestans at different spatial scales. Acta Trop. 2014;140:10-18.
http://dx.doi.org/10.1016/j.actatropica.2014.07.014