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Sulfation, a post-translational modification which plays a key role in various biological processes, is inhibited by competition with chlorate. In Trypanosoma cruzi, the agent of Chagas' disease, sulfated structures have been described as part of glycolipids and we have reported sulfated high-mannose type oligosaccharides in the C-T domain of the cruzipain (Cz) glycoprotein. However, sulfation pathways have not been described yet in this parasite. Herein, we studied the effect of chlorate treatment on T. cruzi with the aim to gain some knowledge about sulfation metabolism and the role of sulfated molecules in this parasite. In chlorate-treated epimastigotes, immunoblotting with anti-sulfates enriched Cz IgGs (AS-enriched IgGs) showed Cz undersulfation. Accordingly, a Cz mobility shift toward higher isoelectric points was observed in 2D-PAGE probed with anti-Cz antibodies. Ultrastructural membrane abnormalities and a significant decrease of dark lipid reservosomes were shown by electron microscopy and a significant decrease in sulfatide levels was confirmed by TLC/UV-MALDI-TOF-MS analysis. Altogether, these results suggest T. cruzi sulfation occurs via PAPS. Sulfated epitopes in trypomastigote and amastigote forms were evidenced using AS-enriched IgGs by immunoblotting. Their presence on trypomastigotes surface was demonstrated by flow cytometry and IF with Cz/dCz specific antibodies. Interestingly, the percentage of infected cardiac HL-1 cells decreased 40% when using chlorate-treated trypomastigotes, suggesting sulfates are involved in the invasion process. The same effect was observed when cells were pre-incubated with dCz, dC-T or an anti-high mannose receptor (HMR) antibody, suggesting Cz sulfates and HMR are also involved in the infection process by T. cruzi. © 2014 Elsevier B.V.


Documento: Artículo
Título:Effects of chlorate on the sulfation process of trypanosoma cruzi glycoconjugates. Implication of parasite sulfates in cellular invasion
Autor:Ferrero, M.R.; Soprano, L.L.; Acosta, D.M.; García, G.A.; Esteva, M.I.; Couto, A.S.; Duschak, V.G.
Filiación:Area de Bioquímica de Proteínas y Glicobiología de Parásitos, Departamento de Investigación, Instituto Nacional de Parasitologia, Dr Mario Fatala Chaben, ANLIS-Malbrán, Ministerio de Salud de la Nación, Paseo Colòn 568, CABA (1063), Buenos Aires, Argentina
CIHIDECAR, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Cruzipain; Invasion process; Sulfation; Sulfoglycosphingolipids; Trypanosoma cruzi; chlorate; cruzipain; cruzipain antibody; enzyme antibody; glycoconjugate; immunoglobulin G; mannose receptor; mannose receptor antibody; receptor antibody; sulfatide; unclassified drug; chlorate; cysteine proteinase; glycoconjugate; sulfate; antibody; electron microscopy; lipid; membrane; metabolism; parasite; amastigote; animal cell; antibody specificity; article; cell invasion; cell organelle; cell ultrastructure; controlled study; electron microscopy; epimastigote; flow cytometry; gel mobility shift assay; host parasite interaction; immunoblotting; isoelectric point; lipid reservosome; matrix assisted laser desorption ionization time of flight mass spectrometry; nonhuman; polyacrylamide gel electrophoresis; sulfation; thin layer chromatography; Trypanosoma cruzi; trypomastigote; ultraviolet spectroscopy; animal; cell line; drug effects; endocytosis; heart muscle cell; human; mass spectrometry; metabolism; parasitology; physiology; protein processing; rabbit; Trypanosoma cruzi; two dimensional gel electrophoresis; Animals; Cell Line; Chlorates; Cysteine Endopeptidases; Electrophoresis, Gel, Two-Dimensional; Endocytosis; Glycoconjugates; Humans; Immunoblotting; Isoelectric Point; Metabolic Networks and Pathways; Microscopy, Electron; Myocytes, Cardiac; Protein Processing, Post-Translational; Rabbits; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfates; Trypanosoma cruzi
Página de inicio:161
Página de fin:173
Título revista:Acta Tropica
Título revista abreviado:Acta Trop.
CAS:chlorate, 14866-68-3; cruzipain; immunoglobulin G, 97794-27-9; cysteine proteinase, 37353-41-6; sulfate, 14808-79-8; Chlorates; cruzipain; Cysteine Endopeptidases; Glycoconjugates; Sulfates


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---------- APA ----------
Ferrero, M.R., Soprano, L.L., Acosta, D.M., García, G.A., Esteva, M.I., Couto, A.S. & Duschak, V.G. (2014) . Effects of chlorate on the sulfation process of trypanosoma cruzi glycoconjugates. Implication of parasite sulfates in cellular invasion. Acta Tropica, 137, 161-173.
---------- CHICAGO ----------
Ferrero, M.R., Soprano, L.L., Acosta, D.M., García, G.A., Esteva, M.I., Couto, A.S., et al. "Effects of chlorate on the sulfation process of trypanosoma cruzi glycoconjugates. Implication of parasite sulfates in cellular invasion" . Acta Tropica 137 (2014) : 161-173.
---------- MLA ----------
Ferrero, M.R., Soprano, L.L., Acosta, D.M., García, G.A., Esteva, M.I., Couto, A.S., et al. "Effects of chlorate on the sulfation process of trypanosoma cruzi glycoconjugates. Implication of parasite sulfates in cellular invasion" . Acta Tropica, vol. 137, 2014, pp. 161-173.
---------- VANCOUVER ----------
Ferrero, M.R., Soprano, L.L., Acosta, D.M., García, G.A., Esteva, M.I., Couto, A.S., et al. Effects of chlorate on the sulfation process of trypanosoma cruzi glycoconjugates. Implication of parasite sulfates in cellular invasion. Acta Trop. 2014;137:161-173.