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Abstract:

Snails of the Family Lymnaeidae act as an intermediate hosts of Fasciola hepatica worldwide. The taxonomy of lymnaeid species is relevant for epidemiological studies and molecular strategies are increasingly used for that purpose. This work presents the first report of a real-time PCR approach used to identify the most important lymnaeid species in the Southern Cone of South America. Species discrimination is based on the sequence polymorphism located within the helix E10-1 of the variable region V2 of the 18S rRNA genes, which yields amplicons with clearly different melting temperatures. This procedure minimises the risk of carry-over contamination because it does not require post-PCR manipulations, and the whole protocol can be completed in less than 4 h with a single snail foot as starting material. This method was successfully carried out in a blind study that included a panel of 20 Galba truncatula, 5 Lymnaea viatrix, 5 Lymnaea diaphana and 5 Pseudosuccinea columella specimens from different endemic areas for fasciolosis. This molecular approach constitutes a key laboratory tool complementing ecological studies that ultimately will promote more efficient control strategies. © 2008 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Real-time PCR strategy for rapid discrimination among main lymnaeid species from Argentina
Autor:Duffy, T.; Kleiman, F.; Pietrokovsky, S.; Issia, L.; Schijman, A.G.; Wisnivesky-Colli, C.
Filiación:Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, 1427 Buenos Aires, Argentina
Unidad de Ecología de Reservorios y Vectores de Parásitos, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:18S rDNA; Fasciolosis; Galba truncatula; Lymnaea diaphana; Lymnaea viatrix; Lymnaeidae; Pseudosuccinea columella; Real-time PCR; ribosome RNA; endemic species; flatworm; identification method; intermediate host; polymerase chain reaction; polymorphism; real time; snail; taxonomy; amplicon; Argentina; article; control strategy; endemic disease; Fasciola hepatica; Galba truncatula; gene location; genetic manipulation; genetic polymorphism; intermediate host; Lymnaea; Lymnaea diaphana; Lymnaea viatrix; melting point; nonhuman; nucleotide sequence; Pseudosuccinea columella; real time polymerase chain reaction; species comparison; species differentiation; species identification; taxonomy; Animals; Argentina; Lymnaea; Phylogeny; Polymerase Chain Reaction; RNA, Ribosomal, 18S; Sequence Analysis, DNA; Argentina; South America; Diaphana; Fasciola hepatica; Galba truncatula; Gastropoda; Lymnaea; Lymnaea viatrix; Lymnaeidae; Pseudosuccinea columella
Año:2009
Volumen:109
Número:1
Página de inicio:1
Página de fin:4
DOI: http://dx.doi.org/10.1016/j.actatropica.2008.08.003
Título revista:Acta Tropica
Título revista abreviado:Acta Trop.
ISSN:0001706X
CODEN:ACTRA
CAS:RNA, Ribosomal, 18S
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v109_n1_p1_Duffy

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Citas:

---------- APA ----------
Duffy, T., Kleiman, F., Pietrokovsky, S., Issia, L., Schijman, A.G. & Wisnivesky-Colli, C. (2009) . Real-time PCR strategy for rapid discrimination among main lymnaeid species from Argentina. Acta Tropica, 109(1), 1-4.
http://dx.doi.org/10.1016/j.actatropica.2008.08.003
---------- CHICAGO ----------
Duffy, T., Kleiman, F., Pietrokovsky, S., Issia, L., Schijman, A.G., Wisnivesky-Colli, C. "Real-time PCR strategy for rapid discrimination among main lymnaeid species from Argentina" . Acta Tropica 109, no. 1 (2009) : 1-4.
http://dx.doi.org/10.1016/j.actatropica.2008.08.003
---------- MLA ----------
Duffy, T., Kleiman, F., Pietrokovsky, S., Issia, L., Schijman, A.G., Wisnivesky-Colli, C. "Real-time PCR strategy for rapid discrimination among main lymnaeid species from Argentina" . Acta Tropica, vol. 109, no. 1, 2009, pp. 1-4.
http://dx.doi.org/10.1016/j.actatropica.2008.08.003
---------- VANCOUVER ----------
Duffy, T., Kleiman, F., Pietrokovsky, S., Issia, L., Schijman, A.G., Wisnivesky-Colli, C. Real-time PCR strategy for rapid discrimination among main lymnaeid species from Argentina. Acta Trop. 2009;109(1):1-4.
http://dx.doi.org/10.1016/j.actatropica.2008.08.003