Artículo
Abstract:
Studies were carried out to elucidate if in the hexachlorobenzene (HCB) pophyria, total, nonhaem and haem iron contents in liver are altered and if any relation exists between these alterations and the hepatic porphyrinogen carboxy-lyase (PCL) decrease in rats treated with the drug. It was observed that in porphyric livers total and non-haem iron levels increased significantly as a consequence of HCB intoxication while this treatment produced a non significant decrease in the haem iron content. Enzymic preparations of porphyric livers filtered through Sephadex G-25 columns which separate the free iron and that has a content of iron-protein greater than those in normals, exhibited a strong inhibition of PCL. Chelating agents, α α' bipyridyl and 8-hydroxyquinoline do not revert such inhibition. The effect in vitro of ferritin, haemin and inorganic iron at different concentrations of normal PCL activity was also assayed. So, it could be observed that inorganic iron and haemin produce slight inhibition of PCL when added in concentrations higher than those corresponding to a porphyric liver (0.08mM and 10-6 M, respectively, as mean in the incubation media). So, they have no physiological significance. Ferritin does not modify the decarboxylation process. From these results it arises that iron does not play a direct role in the decrease of PCL activity in the experimental porphyria by HCB, not being the inhibitor made evident by heating assays. Iron could perhaps stimulate the metabolization of HCB, giving rise to active metabolite.
Registro:
| Documento: | Artículo |
| Título: | The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease |
| Autor: | Wainstok De Calmanovici, R.; Rios De Molina del, M.C.; San Martin De Viale, L.C. |
| Filiación: | Laboratorio de Porfirias Experimentales y Metabolismo del Hemo, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Nunez, 1428 Buenos Aires, Argentina |
| Palabras clave: | ferritin; heme; hexachlorobenzene; iron; uroporphyrinogen decarboxylase; animal experiment; blood and hemopoietic system; intoxication; liver; nonhuman; porphyria; rat; Animal; Carboxy-Lyases; Chlorobenzenes; Disease Models, Animal; Female; Heme; Hexachlorobenzene; Iron; Liver; Porphyria; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't; Uroporphyrinogen Decarboxylase |
| Año: | 1985 |
| Volumen: | 35 |
| Número: | 4 |
| Página de inicio: | 481 |
| Página de fin: | 491 |
| Título revista: | Acta Physiologica et Pharmacologica Latinoamericana |
| Título revista abreviado: | ACTA PHYSIOL. PHARMACOL. LATINOAM. |
| ISSN: | 00016764 |
| CODEN: | APPLE |
| CAS: | ferritin, 9007-73-2; heme, 14875-96-8; hexachlorobenzene, 118-74-1, 55600-34-5; iron, 14093-02-8, 53858-86-9, 7439-89-6; uroporphyrinogen decarboxylase, 9024-70-8; Carboxy-Lyases, EC 4.1.1.; Chlorobenzenes; Heme, 14875-96-8; Hexachlorobenzene, 118-74-1; Iron, 7439-89-6; Uroporphyrinogen Decarboxylase, EC 4.1.1.37 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00016764_v35_n4_p481_WainstokDeCalmanovici |
Citas:
---------- APA ----------
Wainstok De Calmanovici, R., Rios De Molina del, M.C. & San Martin De Viale, L.C. (1985) . The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease. Acta Physiologica et Pharmacologica Latinoamericana, 35(4), 481-491.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00016764_v35_n4_p481_WainstokDeCalmanovici [ ]
---------- CHICAGO ----------
Wainstok De Calmanovici, R., Rios De Molina del, M.C., San Martin De Viale, L.C. "The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease" . Acta Physiologica et Pharmacologica Latinoamericana 35, no. 4 (1985) : 481-491.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00016764_v35_n4_p481_WainstokDeCalmanovici [ ]
---------- MLA ----------
Wainstok De Calmanovici, R., Rios De Molina del, M.C., San Martin De Viale, L.C. "The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease" . Acta Physiologica et Pharmacologica Latinoamericana, vol. 35, no. 4, 1985, pp. 481-491.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00016764_v35_n4_p481_WainstokDeCalmanovici [ ]
---------- VANCOUVER ----------
Wainstok De Calmanovici, R., Rios De Molina del, M.C., San Martin De Viale, L.C. The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease. ACTA PHYSIOL. PHARMACOL. LATINOAM. 1985;35(4):481-491.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00016764_v35_n4_p481_WainstokDeCalmanovici [ ]
