Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

In the present work we studied different characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes (CIC). Twenty-five insulindependent (IDD) and 25 non-insulin-dependent diabetic (NIDD) patients were evaluated. Each group was then subdivided according to the presence or absence of microvascular complications (MC). We found that the chemiluminescence (CL) emitted by opsonized zymosan (Zop) stimulated neutrophils in IDD and NIDD patients was significantly increased when compared to healthy subjects (p<0.01 and p<0.02, respectively). The CL values were correlated to CIC levels and both parameters were related to the presence of MC. On the other hand, the percentage of neutrophils capable of reducing nitroblue tetrazolium was diminished in the two groups of diabetic patients (p<0.05 for IDD and p<0.01 for NIDD). The percentage of neutrophils with functional C3b receptors was normal in diabetic patients; however, the proportion of phagocytic cells through Fc receptors was significantly decreased in both types of patients (p<0.05 and p<0.01 for IDD and NIDD, respectively). Furthermore, the number of granulocytes with immune complexes (IC) bound to their cell surface was increased in diabetics. We suggest that the increase of CIC level may produce an increase in IC binding to the neutrophil membrane. These IC could block the Fc receptors, diminish phagocytic capacity and, simultaneously, stimulate the release of toxic oxygen products, thus contributing to produce tissue damage. © 1988 Casa Editrice «il Ponte».

Registro:

Documento: Artículo
Título:Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes
Autor:Barañao, R.I.; Rumi, L.S.; Tesone, P.A.; Foglia, V.G.
Filiación:Instituto de Biologia y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:Chemiluminescence; Circulating immune complexes; Neurophils; Phagocytic activity; Fc receptor; adult; antigen antibody complex; chemoluminescence; clinical article; diabetes mellitus; female; human; male; neutrophil; phagocytosis; Antigen-Antibody Complex; Blood Glucose; Diabetes Mellitus, Insulin-Dependent; Diabetes Mellitus, Non-Insulin-Dependent; Human; Luminescence; Neutrophils; Phagocytosis
Año:1988
Volumen:25
Número:1
Página de inicio:13
Página de fin:23
DOI: http://dx.doi.org/10.1007/BF02581241
Título revista:Acta Diabetologica Latina
Título revista abreviado:Acta diabet. lat
ISSN:00015563
CODEN:ADILA
CAS:Antigen-Antibody Complex; Blood Glucose
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00015563_v25_n1_p13_Baranao

Referencias:

  • Andersen, B., Amirauld, H.J., Important variables in granulocyte chemiluminescence (1979) Proc. Soc. exp. Biol. (N.Y.), 162, p. 139
  • Archibald, A.C., Cheung, K., Robinson, M.F., The interaction of lymphocyte surface-bound immune complexes and neutrophils (1983) J. Immunol., 131, p. 207
  • Baehner, R.L., Boxer, L.A., Davis, J., The biochemical basis of nitroblue tetrazolium reduction in normal human and chronic granulomatous disease polymorphonuclear leukocytes (1976) Blood, 48, p. 309
  • Barañao, R.I., Garberi, J.C., Tesone, P.A., Rumi, L.S., Evaluation of neutrophil activity and circulating immune complexes levels in diabetic patients (1987) Hormone metabol. Res., 19, p. 371
  • Barañao, R.I., Tesone, P.A., Chasseing, N.A., Mendelson, M., Rumi, L.S., Circulating immune complexes and B lymphocytes in diabetic patients with and without microangiopathy (1983) Acta physiol. pharmacol. lat.-amer., 35, p. 145
  • Camussi, G., Tetta, C., Cappio, E.C., Detection of immune complexes on the surface of polymorphonuclear neutrophils (1979) Int. Arch. Allergy, 58, p. 135
  • Cheson, B.D., Christensen, R.L., Sperling, R., Kohler, B.E., Babior, B.M., The origin of the chemiluminescence of phagocytosing granulocytes (1976) J. clin. Invest., 58, p. 789
  • Cheung, K., Archibald, A.C., Robinson, M.F., The origin of chemiluminescence produced by neutrophils stimulated by opsonized zymosan (1983) J. Immunol., 130, p. 2324
  • Creighton, W.D., Lambert, P.H., Miescher, P.A., Detection of antibodies and soluble antigenantibody complexes by precipitation with polyethylene glycol (1973) J. Immunol., 111, p. 1219
  • Di Mario, U., Galfo, C., Ventriglia, L., Franco, M., Schena, F.P., Valesini, G., Natali, P.G., Celada, F., Immune complexes in diabetics with microangiopathy. A collaborative study for the evaluation of 9 different techniques (1981) Diabetologia, 21, p. 264
  • Di Mario, U., Iavicoli, M., Andreani, D., Circulating immune complexes in diabetes mellitus (1980) Diabetologia, 19, p. 89
  • Dziatowiak, H., Kowalska, M., Denys, A., Phagocytic and bactericidal activity of granulocytes in diabetic children (1982) Diabetes, 31, p. 1041
  • Easmann, V., The polymorphonuclear leukocyte in diabetes mellitus (1983) J. clin. Chem. clin. Biochem., 21, p. 561
  • Easmon C. S. F., Cole P. J.: Luminol-dependent chemiluminescence. In: Thompson R. A. (Ed.): Techniques in clinical immunology. Blackwell Scientific Publications, 1981; p. 294; Griffin, F.M., Effects of soluble immune complexes on FC receptor and C3b receptor mediated phagocytosis by macrophages (1980) J. exp. Med., 152, p. 905
  • Hed, J., Stendhal, O., Differences in the ingestion mechanism of IgG and C3b particles in phagocytosis by neutrophils (1982) Immunology, 45, p. 727
  • Henson, P.M., Oades, Z.B., Stimulation of human neutrophils by soluble immunoglobulin aggregates. Secretion of granule constituents and increased oxidation of glucose (1976) J. clin. Invest., 56, p. 1053
  • Humbert, J.B., Gross, G.P., Vatter, A.E., Hathaway, W.E., Nitroblue tetrazolium reduction by neutrophils. Biochemical and ultrastructural effects of methylene blue (1973) J Lab Clin Med, 82, p. 20
  • Iavicoli, M., Di Mario, U., Pozzilli, P., Canalese, J., Ventriglia, L., Galfo, C., Andreani, D., Impaired phagocytic function and increased immune complexes in diabetics with severe microangiopathy (1982) Diabetes, 31, p. 7
  • Johnston, R.B., Lehmeyer, J.E., Elaboration of toxic oxygen byproducts by neutrophils in a model of immune complex disease (1971) J. clin. Invest., 57, p. 836
  • Kilpatrick, J.M., Virelia, G., The role of circulating immune complexes in the pathogenesis of diabetes mellitus (1982) Acta diabetol. lat., 19, p. 107
  • Kurlander, R.J., Reversible and irreversible loss of FC receptor function of human monocytes as a consequence of interaction with immunoglobulin G (1980) J. clin. Invest., 66, p. 773
  • Lawrence, S., Charlesworth, J.A., Pussell, B.A., Campbell, L.V., Kotowicz, M.A., Factors influencing reticulophagocytic function in insulin-treated diabetes (1984) Diabetes, 33, p. 813
  • Matkovics, B., Varga, S.I., Szabó, L., Witas, H., The effect of diabetes on the activities of the peroxide metabolism enzymes (1982) Hormone metabol. Res., 14, p. 77
  • Motocu, M., Duma, V., Dutu, A.L., Rusu, M.L., Dutu, D., Cristea, A., Circulating immune complexes in diabetes mellitus (1981) Diabetologia, 21, p. 307
  • Nath, N., Chari, S.N., Rathi, A.B., Superoxide dismutase in diabetic polymorphonuclear leukocytes (1984) Diabetes, 33, p. 586
  • Niwa, Y., Sakane, T., Shingu, M., Yokoyama, M.M., Effect of stimulated neutrophils from the synovial fluid of patients with rheumatoid arthritis on lymphocytes. A possible role of increased oxygen radicals generated by neutrophils (1983) J. clin. Immunol., 3, p. 228
  • Sagone, A.L., Mendelson, D.S., Metz, E.N., The effect of sodium azide on the chemiluminescence of granulocytes, evidence for the generation of multiple oxygen radicals (1977) J. Lab. clin. Med., 89, p. 1333
  • Smith, R.L., Weidemann, M.J., Reactive oxygen production associated with arachidonic acid metabolism by peripheral macrophages (1983) Biochem. biophys. Res. Commun., 97, p. 973
  • Starkebaum, G., Arend, W.P., Neutrophil-binding immunoglobulin G in systemic lupus erythematosus (1979) J. clin. Invest., 64, p. 902
  • Starkebaum, G., Jimenez, R.A.H., Arend, W.P., Effect of immune complexes on human neutrophil phagocytic function (1982) J. Immunol., 128, p. 141
  • Starkebaum, G., Stevens, D.L., Henry, C., Gavin, S.E., Stimulation of human neutrophil chemiluminescence by soluble immune complexes and antibodies to neutrophils (1981) J. Lab. clin. Med., 98, p. 280
  • Sterfalaar, J.W., Tenkate, J.W., Nap, M., Swaak, A.J.G., Degraffreistma, C.B., Van Elven, E.D.H., Felkamp-Vroom, T.M., Immune complex detection by immune fluorescence on polymorphonuclear leukocytes (1977) Clin. exp. Immunol., 27, p. 391
  • Subbaiah, P.V., Bagdade, J.D., Host defence in diabetes mellitus: defective membrane synthesis during phagocytosis (1982) Hormone metabol. Res., 14, p. 445
  • Thaiss, E., Batsford, S., Mihatsch, M.J., Heistz, P.V., Bitter-Suermann, D., Vogt, A., Mediator systems in a passive model of in situ immune complex glomerulonephritis (1986) Lab. Invest., 54, p. 624
  • Trush, M.A., Wilson, M.E., Van Dyke, K., The generation of chemiluminescence (CL) by phagocytic cells (1978) Meth. Enzymol., 57, p. 462
  • Virella, G., Wohltmann, H., Sagel, J., Lopes-Virelia, M.F.L., Kilpatrick, M., Phillips, C., Colwell, J., Soluble immune complexes in patients with diabetes mellitus: detection and pathological significance (1981) Diabetologia, 21, p. 184

Citas:

---------- APA ----------
Barañao, R.I., Rumi, L.S., Tesone, P.A. & Foglia, V.G. (1988) . Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes. Acta Diabetologica Latina, 25(1), 13-23.
http://dx.doi.org/10.1007/BF02581241
---------- CHICAGO ----------
Barañao, R.I., Rumi, L.S., Tesone, P.A., Foglia, V.G. "Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes" . Acta Diabetologica Latina 25, no. 1 (1988) : 13-23.
http://dx.doi.org/10.1007/BF02581241
---------- MLA ----------
Barañao, R.I., Rumi, L.S., Tesone, P.A., Foglia, V.G. "Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes" . Acta Diabetologica Latina, vol. 25, no. 1, 1988, pp. 13-23.
http://dx.doi.org/10.1007/BF02581241
---------- VANCOUVER ----------
Barañao, R.I., Rumi, L.S., Tesone, P.A., Foglia, V.G. Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes. Acta diabet. lat. 1988;25(1):13-23.
http://dx.doi.org/10.1007/BF02581241