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Abstract:

Triatoma infestans, the main vector of Chagas disease in the southern cone countries, is the principal target of a regional elimination program. A better understanding of its dispersal, sources of reinfestation, and insecticide resistance is key to an effective control program. To address such problems, we identified and characterized 13 microsatellite loci of T. infestans. For each locus, primer sequences and PCR conditions are presented. Allele variability and frequency were analyzed in 59 T. infestans specimens from different rural communities in northwestern Argentina; nine loci were considered suitable for population genetic studies. Departure from Hardy-Weinberg equilibrium was detected in 10/13 loci with FIS values ranging from 0.04 to 0.91, indicating heterozygote deficit and a possible grade of sub-structure in the sample analyzed. Presence of null alleles in some loci cannot be discarded. The present work provides a promising tool to develop a population genetic study of natural populations of T. infestans in tandem with field studies and analyses of bug dispersal and the reinfestation process. © 2005 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae)
Autor:Marcet, P.L.; Lehmann, T.; Groner, G.; Gürtler, R.E.; Kitron, U.; Dotson, E.M.
Filiación:Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Pab II-2 Piso, 1428 Buenos Aires, Argentina
Center for Disease Control, Division of Parasitic Diseases, Entomology Branch, 4770 Buford Hwy. NE, Chamblee, GA 30341, United States
College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Illinois 2001 S. Lincoln Ave., Urbana, IL 61802, United States
Palabras clave:Chagas disease; Microsatellites; Reinfestation; Triatoma infestans; allele; article; Chagas disease; controlled study; disease carrier; DNA library; gene frequency; gene locus; gene sequence; genetic variability; microsatellite marker; nonhuman; nucleotide sequence; parasite identification; polymerase chain reaction; priority journal; Triatoma infestans; Animals; Chagas Disease; Insect Vectors; Microsatellite Repeats; Triatoma; Heteroptera; Reduviidae; Triatoma infestans
Año:2006
Volumen:6
Número:1
Página de inicio:32
Página de fin:37
DOI: http://dx.doi.org/10.1016/j.meegid.2005.01.002
Título revista:Infection, Genetics and Evolution
Título revista abreviado:Infec. Genet. Evol.
ISSN:15671348
CODEN:IGENC
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v6_n1_p32_Marcet

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Citas:

---------- APA ----------
Marcet, P.L., Lehmann, T., Groner, G., Gürtler, R.E., Kitron, U. & Dotson, E.M. (2006) . Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae). Infection, Genetics and Evolution, 6(1), 32-37.
http://dx.doi.org/10.1016/j.meegid.2005.01.002
---------- CHICAGO ----------
Marcet, P.L., Lehmann, T., Groner, G., Gürtler, R.E., Kitron, U., Dotson, E.M. "Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae)" . Infection, Genetics and Evolution 6, no. 1 (2006) : 32-37.
http://dx.doi.org/10.1016/j.meegid.2005.01.002
---------- MLA ----------
Marcet, P.L., Lehmann, T., Groner, G., Gürtler, R.E., Kitron, U., Dotson, E.M. "Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae)" . Infection, Genetics and Evolution, vol. 6, no. 1, 2006, pp. 32-37.
http://dx.doi.org/10.1016/j.meegid.2005.01.002
---------- VANCOUVER ----------
Marcet, P.L., Lehmann, T., Groner, G., Gürtler, R.E., Kitron, U., Dotson, E.M. Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae). Infec. Genet. Evol. 2006;6(1):32-37.
http://dx.doi.org/10.1016/j.meegid.2005.01.002