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Abstract:

Evidence has been obtained showing that transport of S-aminolevulinic acid (ALA), a precursor of porphyrin biosynthesis in Saccharomyces cerevisiae, is mediated by the γ-aminobutyric acid (GABA)-specific permease, UGA4. In yeast GABA is also incorporated by the general amino acid permease (GAP1) and the specific proline permease (PUT4). The aim of the present work was to carry out a comparative study on the regulation of ALA and GABA transport to confirm our proposal that both compounds share the UGA4 permease. ALA and GABA uptake were measured in cells grown on minimal media with different carbon and/or nitrogen sources. To study the effect of the carbon source on UGA4 permease, ALA and GABA incorporation mere measured in D27 strain, lacking GAP1 permease, and grown in proline as the sole nitrogen source, so the activity of PUT4 permease was negligible. The effect of the nitrogen source on UGA4 permease was studied measuring ALA and GABA uptake rates in cells from media with ammonium, proline and urea as nitrogen sources. It was found that the regulation by the carbon source was similar on ALA and GABA transport; they depend equally on the energetic conditions of the cells. Moreover, regulation by the nitrogen source on ALA and GABA uptake was also similar, and identical to that described already for UGA4 permease. These results are further evidence that both compounds, ALA and GABA, share the GABA-specific permease, UGA4.

Registro:

Documento: Artículo
Título:Carbon and nitrogen sources regulate δ-aminolevulinic acid and γ-aminobutyric acid transport in Saccharomyces cerevisiae
Autor:Garcí, S.C.; Moretti, M.B.; Ramos, E.; Batlle, A.
Filiación:Ctro. de Invest. Sobre Porfirinas P., Ciudad Universitaria, Pabellón II, 2o Piso, 1428 Buenos Aires, Argentina
Departimento. de Bioquim., Facultad de Medicina, UBA, Paraguay 2155, 5o Piso, 1121 Buenos Aires, Argentina
Viamonte 1881, 1056 Buenos Aires, Argentina
Palabras clave:ALA; GABA; Saccharomyces cerevisiae; Transport regulation; UGA4 permease; 4 aminobutyric acid; aminolevulinic acid; carbon; nitrogen; permease; 4 aminobutyric acid uptake; article; controlled study; nonhuman; saccharomyces cerevisiae; Aminolevulinic Acid; Biological Transport; Carbon; Culture Media; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid; Kinetics; Membrane Transport Proteins; Nitrogen; Organic Anion Transporters; Proline; Quaternary Ammonium Compounds; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Urea; Saccharomyces cerevisiae
Año:1997
Volumen:29
Número:8-9
Página de inicio:1097
Página de fin:1101
DOI: http://dx.doi.org/10.1016/S1357-2725(97)00047-2
Título revista:International Journal of Biochemistry and Cell Biology
Título revista abreviado:INT. J. BIOCHEM. CELL BIOL.
ISSN:13572725
CODEN:IJBBF
CAS:Aminolevulinic Acid, 106-60-5; Carbon, 7440-44-0; Culture Media; GABA permease, 69913-01-5; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid, 56-12-2; Membrane Transport Proteins; Nitrogen, 7727-37-9; Organic Anion Transporters; Proline, 147-85-3; Quaternary Ammonium Compounds; Saccharomyces cerevisiae Proteins; UGA4 protein, S cerevisiae; Urea, 57-13-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v29_n8-9_p1097_Garci

Referencias:

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Citas:

---------- APA ----------
Garcí, S.C., Moretti, M.B., Ramos, E. & Batlle, A. (1997) . Carbon and nitrogen sources regulate δ-aminolevulinic acid and γ-aminobutyric acid transport in Saccharomyces cerevisiae. International Journal of Biochemistry and Cell Biology, 29(8-9), 1097-1101.
http://dx.doi.org/10.1016/S1357-2725(97)00047-2
---------- CHICAGO ----------
Garcí, S.C., Moretti, M.B., Ramos, E., Batlle, A. "Carbon and nitrogen sources regulate δ-aminolevulinic acid and γ-aminobutyric acid transport in Saccharomyces cerevisiae" . International Journal of Biochemistry and Cell Biology 29, no. 8-9 (1997) : 1097-1101.
http://dx.doi.org/10.1016/S1357-2725(97)00047-2
---------- MLA ----------
Garcí, S.C., Moretti, M.B., Ramos, E., Batlle, A. "Carbon and nitrogen sources regulate δ-aminolevulinic acid and γ-aminobutyric acid transport in Saccharomyces cerevisiae" . International Journal of Biochemistry and Cell Biology, vol. 29, no. 8-9, 1997, pp. 1097-1101.
http://dx.doi.org/10.1016/S1357-2725(97)00047-2
---------- VANCOUVER ----------
Garcí, S.C., Moretti, M.B., Ramos, E., Batlle, A. Carbon and nitrogen sources regulate δ-aminolevulinic acid and γ-aminobutyric acid transport in Saccharomyces cerevisiae. INT. J. BIOCHEM. CELL BIOL. 1997;29(8-9):1097-1101.
http://dx.doi.org/10.1016/S1357-2725(97)00047-2