Artículo

Abstract:

Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis. © 2010 American Society for Mass Spectrometry.

Registro:

Documento: Artículo
Título:UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
Autor:Bultel, L.; Landoni, M.; Grand, E.; Couto, A.S.; Kovensky, J.
Filiación:Laboratoire des Glucides CNRS UMR 6219, Institut de Chimie de Picardie, Université de Picardie Jules Verne, Amiens, France
CIHIDECAR (CONICET), Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:Controlled degradation; Cyano-4-hydroxycinnamic acids; Dihydroxybenzoic acids; Harmane; High-performance anion-exchange chromatography; MALDI-TOF; MALDI-TOF mass spectrometry; Matrix assisted laser desorption/ionization; New tools; Nitrous acid; Structural determination; Sulfate groups; Chromatographic analysis; Chromatography; Degradation; Desorption; Inorganic acids; Mass spectrometry; Oligosaccharides; Polysaccharides; Structural analysis; Ultraviolet lasers; Negative ions; alpha cyano 4 hydroxycinnamic acid; benzoic acid derivative; beta carboline; dihydroxybenzoic acid; heparin; nitrous acid; oligosaccharide; sulfate; unclassified drug; anion exchange chromatography; article; carbohydrate analysis; chemical structure; matrix assisted laser desorption ionization time of flight mass spectrometry; purification; structure analysis; ultraviolet spectroscopy; Carbohydrate Sequence; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Heparin; Molecular Sequence Data; Molecular Structure; Nitrous Acid; Oligosaccharides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet; Time Factors
Año:2010
Volumen:21
Número:1
Página de inicio:178
Página de fin:190
DOI: http://dx.doi.org/10.1016/j.jasms.2009.09.025
Título revista:Journal of the American Society for Mass Spectrometry
Título revista abreviado:J. Am. Soc. Mass Spectrom.
ISSN:10440305
CODEN:JAMSE
CAS:alpha cyano 4 hydroxycinnamic acid, 28166-41-8; beta carboline, 244-63-3; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; nitrous acid, 7782-77-6; sulfate, 14808-79-8; Heparin, 9005-49-6; Nitrous Acid, 7782-77-6; Oligosaccharides
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Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v21_n1_p178_Bultel

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Citas:

---------- APA ----------
Bultel, L., Landoni, M., Grand, E., Couto, A.S. & Kovensky, J. (2010) . UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography. Journal of the American Society for Mass Spectrometry, 21(1), 178-190.
http://dx.doi.org/10.1016/j.jasms.2009.09.025
---------- CHICAGO ----------
Bultel, L., Landoni, M., Grand, E., Couto, A.S., Kovensky, J. "UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography" . Journal of the American Society for Mass Spectrometry 21, no. 1 (2010) : 178-190.
http://dx.doi.org/10.1016/j.jasms.2009.09.025
---------- MLA ----------
Bultel, L., Landoni, M., Grand, E., Couto, A.S., Kovensky, J. "UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography" . Journal of the American Society for Mass Spectrometry, vol. 21, no. 1, 2010, pp. 178-190.
http://dx.doi.org/10.1016/j.jasms.2009.09.025
---------- VANCOUVER ----------
Bultel, L., Landoni, M., Grand, E., Couto, A.S., Kovensky, J. UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography. J. Am. Soc. Mass Spectrom. 2010;21(1):178-190.
http://dx.doi.org/10.1016/j.jasms.2009.09.025