Artículo

Abstract:

The effect of eliminating D-lactate synthesis in poly(3-hydroxybutyrate) (PHB)-accumulating recombinant Escherichia coli (K24K) was analyzed using glycerol as a substrate. K24KL, an ldhA derivative, produced more biomass and had altered carbon partitioning among the metabolic products, probably due to the increased availability of carbon precursors and reducing power. This resulted in a significant increase of PHB and ethanol synthesis and a decrease in acetate production. Cofactor measurements revealed that cultures of K24K and K24KL had a high intracellular NADPH content and that the NADPH/NADP+ ratio was higher than the NADH/NAD+ ratio. The ldhA mutation affected cofactor distribution, resulting in a more reduced intracellular state, mainly due to a further increase in NADPH/NADP+. In 60-h fed-batch cultures, K24KL reached 41.9 g · liter-1 biomass and accumulated PHB up to 63% ± 1% (wt/wt), with a PHB yield on glycerol of 0.41 ± 0.03 g · g-1, the highest reported using this substrate. © 2010, American Society for Microbiology.

Registro:

Documento: Artículo
Título:Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli
Autor:Nikel, P.I.; Giordano, A.M.; De Almeida, A.; Godoy, M.S.; Pettinari, M.J.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactasy Naturales, Ciudad Universitaria-Pabellón 2, Av. Intendente Güiraldes 2160, C1428EGA Buenos Aires, Argentina
Palabras clave:Carbon partitioning; Carbon precursors; Cofactors; D-lactate; Fed-batch cultures; Metabolic products; Poly(3-hydroxybutyrate); Recombinant Escherichia coli; Reducing power; Batch cell culture; Escherichia coli; Ethanol; Substrates; Glycerol; alcohol; carbon; glycerol; hydroxybutyric acid; lactate dehydrogenase; lactic acid; nicotinamide adenine dinucleotide; nicotinamide adenine dinucleotide phosphate; poly(3 hydroxybutyric acid); poly-beta-hydroxybutyrate; polyester; alcohol; biomass; ester; ethanol; fecal coliform; partitioning; recombination; article; biomass; bioreactor; chemistry; Escherichia coli; genetics; growth, development and aging; metabolism; time; Biomass; Bioreactors; Carbon; Escherichia coli; Ethanol; Glycerol; Hydroxybutyrates; Lactate Dehydrogenases; Lactic Acid; Metabolic Networks and Pathways; NAD; NADP; Polyesters; Time Factors; Escherichia coli
Año:2010
Volumen:76
Número:22
Página de inicio:7400
Página de fin:7406
DOI: http://dx.doi.org/10.1128/AEM.02067-10
Título revista:Applied and Environmental Microbiology
Título revista abreviado:Appl. Environ. Microbiol.
ISSN:00992240
CODEN:AEMID
CAS:alcohol, 64-17-5; carbon, 7440-44-0; glycerol, 56-81-5; hydroxybutyric acid, 1320-61-2, 35054-79-6; lactate dehydrogenase, 9001-60-9; lactic acid, 113-21-3, 50-21-5; nicotinamide adenine dinucleotide, 53-84-9; nicotinamide adenine dinucleotide phosphate, 53-59-8; poly(3 hydroxybutyric acid), 26063-00-3; Carbon, 7440-44-0; Ethanol, 64-17-5; Glycerol, 56-81-5; Hydroxybutyrates; Lactate Dehydrogenases, 1.1.-; Lactic Acid, 50-21-5; NAD, 53-84-9; NADP, 53-59-8; Polyesters; poly-beta-hydroxybutyrate, 26063-00-3
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00992240_v76_n22_p7400_Nikel.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v76_n22_p7400_Nikel

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Citas:

---------- APA ----------
Nikel, P.I., Giordano, A.M., De Almeida, A., Godoy, M.S. & Pettinari, M.J. (2010) . Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli. Applied and Environmental Microbiology, 76(22), 7400-7406.
http://dx.doi.org/10.1128/AEM.02067-10
---------- CHICAGO ----------
Nikel, P.I., Giordano, A.M., De Almeida, A., Godoy, M.S., Pettinari, M.J. "Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli" . Applied and Environmental Microbiology 76, no. 22 (2010) : 7400-7406.
http://dx.doi.org/10.1128/AEM.02067-10
---------- MLA ----------
Nikel, P.I., Giordano, A.M., De Almeida, A., Godoy, M.S., Pettinari, M.J. "Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli" . Applied and Environmental Microbiology, vol. 76, no. 22, 2010, pp. 7400-7406.
http://dx.doi.org/10.1128/AEM.02067-10
---------- VANCOUVER ----------
Nikel, P.I., Giordano, A.M., De Almeida, A., Godoy, M.S., Pettinari, M.J. Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli. Appl. Environ. Microbiol. 2010;76(22):7400-7406.
http://dx.doi.org/10.1128/AEM.02067-10