Abstract:
Potato (Solanum tuberosum L.) tuber UDP-glucose:protein transglucosylase (UPTG) (EC 2.4.1.112) is involved in the first of a two-step mechanism proposed for protein-bound α-glucan synthesis by catalyzing the covalent attachment of a single glucose residue to an acceptor protein. The resulting glucosylated 38-kilodalton polypeptide would then serve as a primer for enzymic glucan chain elongation during the second step. In the present report, we describe the fast protein liquid chromatography purification of UPTG from a membrane pellet of potato tuber. An apparently close association of UPTG, phosphorylase, and starch synthase was observed under native conditions during different purification steps. Enrichment of a 38-kilodalton polypeptide was found throughout enzyme purification. It is now shown that the purified UPTG, with an apparent molecular mass of 38 kilodaltons, undergoes self-glucosylation in a UDP-glucose- and Mn2+-dependent reaction. Therefore, it is concluded that UPTG is the enzyme and at the same time the priming protein required for the biogenesis of protein-bound α-glucan in potato tuber.
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Citas:
---------- APA ----------
Ardila, F.J. & Tandecarz, J.S.
(1992)
. Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation. Plant Physiology, 99(4), 1342-1347.
http://dx.doi.org/10.1104/pp.99.4.1342---------- CHICAGO ----------
Ardila, F.J., Tandecarz, J.S.
"Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation"
. Plant Physiology 99, no. 4
(1992) : 1342-1347.
http://dx.doi.org/10.1104/pp.99.4.1342---------- MLA ----------
Ardila, F.J., Tandecarz, J.S.
"Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation"
. Plant Physiology, vol. 99, no. 4, 1992, pp. 1342-1347.
http://dx.doi.org/10.1104/pp.99.4.1342---------- VANCOUVER ----------
Ardila, F.J., Tandecarz, J.S. Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation. Plant Physiol. 1992;99(4):1342-1347.
http://dx.doi.org/10.1104/pp.99.4.1342