Artículo
Abstract:
Chagas disease, a parasitic disease caused by Trypanosoma cruzi, is a major public health burden in poor rural populations of Central and South America and a serious emerging threat outside the endemic region, since the number of infections in non-endemic countries continues to rise. In order to develop more efficient anti-trypanosomal treatments to replace the outdated therapies, new molecular targets need to be explored and new drugs discovered. Trypanosoma cruzi has distinctive structural and functional characteristics with respect to the human host. These exclusive features could emerge as interesting drug targets. In this work, essential and differential protein–protein interactions for the parasite, including the ribosomal P proteins and proteins involved in mRNA processing, were evaluated in a bioluminescence resonance energy transfer-based assay as a starting point for drug screening. Suitable conditions to consider using this simple and robust methodology to screening compounds and natural extracts able to inhibit protein–protein interactions were set in living cells and lysates. © 2018 Springer Science+Business Media, LLC, part of Springer Nature
Registro:
| Documento: | Artículo |
| Título: | Optimization of a Bioluminescence Resonance Energy Transfer-Based Assay for Screening of Trypanosoma cruzi Protein/Protein Interaction Inhibitors |
| Autor: | Mild, J.G.; Fernandez, L.R.; Gayet, O.; Iovanna, J.; Dusetti, N.; Edreira, M.M. |
| Filiación: | Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Buenos Aires, Argentina CONICET - Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina Centre de Recherche en Cancérologie de Marseille (CRCM), INSERM U1068, CNRS UMR 7258, Institut Paoli-Calmettes, Aix Marseille Université, Marseille, France Intendente Guiraldes 2140, Ciudad Universitaria, Pabellón 2, 2do Piso, CM1, Ciudad de Buenos Aires, 1428, Argentina |
| Palabras clave: | BRET; Drug screening; Protein–protein interactions; Trypanosoma cruzi; Bioluminescence; Developing countries; Diagnosis; Energy transfer; Enzyme activity; Health risks; Phosphorescence; Bioluminescence resonance energy transfer; BRET; Drug screening; Functional characteristics; Protein interaction; Screening compounds; Suitable conditions; Trypanosoma cruzi; Drug interactions; antitrypanosomal agent; protein binding; protozoal protein; Central America; chemistry; drug effect; fluorescence resonance energy transfer; HEK293 cell line; human; metabolism; molecular library; pharmacology; preclinical study; procedures; protein analysis; South America; Trypanosoma cruzi; Central America; Drug Evaluation, Preclinical; Fluorescence Resonance Energy Transfer; HEK293 Cells; Humans; Protein Binding; Protein Interaction Maps; Protozoan Proteins; Small Molecule Libraries; South America; Trypanocidal Agents; Trypanosoma cruzi |
| Año: | 2018 |
| Página de inicio: | 1 |
| Página de fin: | 11 |
| DOI: | http://dx.doi.org/10.1007/s12033-018-0078-3 |
| Título revista: | Molecular Biotechnology |
| Título revista abreviado: | Mol. Biotechnol. |
| ISSN: | 10736085 |
| CAS: | Protozoan Proteins; Small Molecule Libraries; Trypanocidal Agents |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10736085_v_n_p1_Mild |
Citas:
---------- APA ----------
Mild, J.G., Fernandez, L.R., Gayet, O., Iovanna, J., Dusetti, N. & Edreira, M.M. (2018) . Optimization of a Bioluminescence Resonance Energy Transfer-Based Assay for Screening of Trypanosoma cruzi Protein/Protein Interaction Inhibitors. Molecular Biotechnology, 1-11.http://dx.doi.org/10.1007/s12033-018-0078-3
---------- CHICAGO ----------
Mild, J.G., Fernandez, L.R., Gayet, O., Iovanna, J., Dusetti, N., Edreira, M.M. "Optimization of a Bioluminescence Resonance Energy Transfer-Based Assay for Screening of Trypanosoma cruzi Protein/Protein Interaction Inhibitors" . Molecular Biotechnology (2018) : 1-11.http://dx.doi.org/10.1007/s12033-018-0078-3
---------- MLA ----------
Mild, J.G., Fernandez, L.R., Gayet, O., Iovanna, J., Dusetti, N., Edreira, M.M. "Optimization of a Bioluminescence Resonance Energy Transfer-Based Assay for Screening of Trypanosoma cruzi Protein/Protein Interaction Inhibitors" . Molecular Biotechnology, 2018, pp. 1-11.http://dx.doi.org/10.1007/s12033-018-0078-3
---------- VANCOUVER ----------
Mild, J.G., Fernandez, L.R., Gayet, O., Iovanna, J., Dusetti, N., Edreira, M.M. Optimization of a Bioluminescence Resonance Energy Transfer-Based Assay for Screening of Trypanosoma cruzi Protein/Protein Interaction Inhibitors. Mol. Biotechnol. 2018:1-11.http://dx.doi.org/10.1007/s12033-018-0078-3
