Abstract:
Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein. © 2009 Springer Science+Business Media, LLC.
Registro:
Documento: |
Artículo
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Título: | Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
Autor: | Distéfano, A.J.; Maldonado, S.; Hopp, H.E.; Del Vas, M. |
Filiación: | Instituto de Biotecnología, CICVyA, CNIA, Instituto Nacional de Tecnología Agropecuaria (INTA), Los Reseros y Las Cabañas s/n, Buenos Aires, 1686 Hurlingham, Argentina Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Ciudad Universitaria, Intendente Güiraldes 2160, Buenos Aires C1428EGA, Argentina
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Palabras clave: | Core protein; Fijivirus; Maize; MRCV; Structural virus proteins; capsid protein; polyclonal antiserum; structural protein; animal experiment; article; controlled study; Escherichia coli; female; immunization; immunoelectron microscopy; immunogold labeling; mal de rio cuarto virus; nonhuman; priority journal; protein expression; protein structure; rabbit; Reovirus; virus genome; virus particle; virus purification; Antibodies, Viral; Capsid Proteins; Escherichia coli; Microscopy, Immunoelectron; Recombinant Fusion Proteins; Reoviridae; Virion; Escherichia coli; Fijivirus; Mal de Rio Cuarto virus; Miridae; Oryctolagus cuniculus; Reoviridae; Zea mays |
Año: | 2009
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Volumen: | 38
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Número: | 3
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Página de inicio: | 455
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Página de fin: | 460
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DOI: |
http://dx.doi.org/10.1007/s11262-009-0345-2 |
Título revista: | Virus Genes
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Título revista abreviado: | Virus Genes
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ISSN: | 09208569
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CODEN: | VIGEE
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CAS: | Antibodies, Viral; Capsid Proteins; Recombinant Fusion Proteins
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09208569_v38_n3_p455_Distefano |
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Citas:
---------- APA ----------
Distéfano, A.J., Maldonado, S., Hopp, H.E. & Del Vas, M.
(2009)
. Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein. Virus Genes, 38(3), 455-460.
http://dx.doi.org/10.1007/s11262-009-0345-2---------- CHICAGO ----------
Distéfano, A.J., Maldonado, S., Hopp, H.E., Del Vas, M.
"Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein"
. Virus Genes 38, no. 3
(2009) : 455-460.
http://dx.doi.org/10.1007/s11262-009-0345-2---------- MLA ----------
Distéfano, A.J., Maldonado, S., Hopp, H.E., Del Vas, M.
"Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein"
. Virus Genes, vol. 38, no. 3, 2009, pp. 455-460.
http://dx.doi.org/10.1007/s11262-009-0345-2---------- VANCOUVER ----------
Distéfano, A.J., Maldonado, S., Hopp, H.E., Del Vas, M. Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein. Virus Genes. 2009;38(3):455-460.
http://dx.doi.org/10.1007/s11262-009-0345-2