Abstract:
In the present work we have been able to demonstrate the phenobarbital and lead exert an inducing effect on the biosynthesis of δ-aminovulenic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the induction effect produced by phenobarbital in this in vitro system. Glucose inhibits the induction of δ-aminolevulinic acid synthase and ferrochelatase. This repression effect can be reversed with increasing concentrations of dibutyryl cyclic AMP. No glucose effect was observed on the phenobarbital- and lead-mediated inductions of cytochrome P-450. The present results add more experimental evidence to support the concept that the last enzyme of the heme pathway is inducible, and as such may have a significant role in regulatory mechanisms of porphyrin and heme biosynthesis. © 1985.
Registro:
Documento: |
Artículo
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Título: | Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead |
Autor: | Canepa, E.T.; Llambias, E.B.C.; Grinstein, M. |
Filiación: | Laboratorio de Porfirinas, Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Núñez, (1428) Buenos Aires Argentina
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Palabras clave: | (Rat hepatocyte); Cytochrome P-450; Enzyme induction; Ferrochelatase; Glucose; δ-Aminolevulinate synthase; 5 aminolevulinate synthase; adrenalin; bucladesine; cycloheximide; cytochrome p450; ferrochelatase; glucose; insulin; lead; phenobarbital; dose response; dose time effect relation; drug response; in vitro study; liver; liver cell; nonhuman; priority journal; 5-Aminolevulinate Synthetase; Animal; Bucladesine; Cells, Cultured; Cycloheximide; Cytochrome P-450 Enzyme System; Enzyme Induction; Epinephrine; Ferrochelatase; Glucose; Hemeproteins; Insulin; Lead; Liver; Lyases; Male; Phenobarbital; Rats; Subcellular Fractions; Support, Non-U.S. Gov't; Theophylline |
Año: | 1985
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Volumen: | 841
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Número: | 2
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Página de inicio: | 186
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Página de fin: | 194
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DOI: |
http://dx.doi.org/10.1016/0304-4165(85)90020-0 |
Título revista: | BBA - General Subjects
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Título revista abreviado: | Biochim. Biophys. Acta Gen. Subj.
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ISSN: | 03044165
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CODEN: | BBGSB
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CAS: | 5 aminolevulinate synthase, 9037-14-3; adrenalin, 51-43-4, 55-31-2, 6912-68-1; bucladesine, 16980-89-5, 362-74-3; cycloheximide, 642-81-9, 66-81-9; cytochrome P450, 9035-51-2; ferrochelatase, 9012-93-5; glucose, 50-99-7, 84778-64-3; insulin, 9004-10-8; lead, 7439-92-1; phenobarbital, 50-06-6, 57-30-7, 8028-68-0; 5-Aminolevulinate Synthetase, EC 2.3.1.37; Bucladesine, 362-74-3; Cycloheximide, 66-81-9; Cytochrome P-450 Enzyme System, 9035-51-2; Epinephrine, 51-43-4; Ferrochelatase, EC 4.99.1.1; Glucose, 50-99-7; Hemeproteins; Insulin, 11061-68-0; Lead, 7439-92-1; Lyases, EC 4.; Phenobarbital, 50-06-6; Theophylline, 58-55-9
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v841_n2_p186_Canepa |
Referencias:
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Citas:
---------- APA ----------
Canepa, E.T., Llambias, E.B.C. & Grinstein, M.
(1985)
. Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead. BBA - General Subjects, 841(2), 186-194.
http://dx.doi.org/10.1016/0304-4165(85)90020-0---------- CHICAGO ----------
Canepa, E.T., Llambias, E.B.C., Grinstein, M.
"Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead"
. BBA - General Subjects 841, no. 2
(1985) : 186-194.
http://dx.doi.org/10.1016/0304-4165(85)90020-0---------- MLA ----------
Canepa, E.T., Llambias, E.B.C., Grinstein, M.
"Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead"
. BBA - General Subjects, vol. 841, no. 2, 1985, pp. 186-194.
http://dx.doi.org/10.1016/0304-4165(85)90020-0---------- VANCOUVER ----------
Canepa, E.T., Llambias, E.B.C., Grinstein, M. Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead. Biochim. Biophys. Acta Gen. Subj. 1985;841(2):186-194.
http://dx.doi.org/10.1016/0304-4165(85)90020-0