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Abstract:

A soluble Ca2+-dependent protein kinase (CDPK) was purified to homogeneity in potato (Solanum tuberosum L.) plants. Potato CDPK was strictly dependent on Ca2+ (one-half maximal activation 0.6 μM) and phosphorylated a wide diversity of substrates, in which Syntide 2 was the best phosphate acceptor (Michaelis constant = 30 μM). The kinase was inhibited by Ca2+- chelating agents, phenotiazine derivatives, and N-(6-aminohexyl)-5-chloro-1- naphthalenesulfonamide (one-half maximal inhibition = 0.25 mM). Polyclonal antibodies directed against the regulatory region of the soybean CDPK recognized a 53-kD polypeptide. In an autophosphorylation assay, this same band was strongly labeled with [γ-32P]ATP in the presence of Ca2+. CDPK activity was high in nontuberized plants, but increased 2.5-fold at the onset of tuber development and was reduced to one-half of its original activity when the tuber had completed formation. In the early stages of tuberization, Ca2+-dependent phosphorylation of endogenous targets (specific bands of 68, 51, and 46 kD) was observed. These polypeptides were not labeled in nontuberizing plants or in completely formed tubers, indicating that this phosphorylation is a stage-specific event. In addition, dephosphorylation of specific polypeptides was detected in tuberizing plants, suggesting the involvement of a phosphatase. Preincubation of crude extracts with phosphatase inhibitors rendered a 100% increase in CDPK activity.

Registro:

Documento: Artículo
Título:Changes in calcium-dependent protein kinase activity during in vitro tuberization in potato
Autor:MacIntosh, G.C.; Ulloa, R.M.; Raíces, M.; Téllez-Iñón, M.T.
Filiación:Inst. Invest. Ing. Genet. y Biol. M., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Glycine max; Solanum tuberosum; Tuberosum
Año:1996
Volumen:112
Número:4
Página de inicio:1541
Página de fin:1550
DOI: http://dx.doi.org/10.1104/pp.112.4.1541
Título revista:Plant Physiology
Título revista abreviado:PLANT PHYSIOL.
ISSN:00320889
CODEN:PLPHA
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00320889_v112_n4_p1541_MacIntosh.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v112_n4_p1541_MacIntosh

Referencias:

  • Bachmann, M., Shiraishi, N., Campbell, W.H., Yoo, B.-C., Harmon, A.C., Huber, S.C., Identification of the major regulatory phosphorylation site as ser-543 in spinach leaf nitrate reductase and its phosphorylation by a calcium-dependent protein kinase in vitro (1996) Plant Cell, 8, pp. 505-517
  • Balamani, V., Veluthambi, K., Poovaiah, B.W., Effect of calcium on tuberization in potato (Solanum tuberosum L.) (1986) Plant Physiol, 80, pp. 856-858
  • Bartfai, T., Preparation of metal chelate complexes and the design of steady-state kinetics experiments involving metal nucleoride complexes (1979) Adv Cyclic Nucleotide Res, 10, pp. 135-167
  • Battey, N.H., Calcium-activated protein kinase from soluble and membrane fractions of maize coleoptiles (1990) Biochem Biophys Res Commun, 170, pp. 17-22
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254
  • Budde, R.J.A., Chollet, R., Regulation of enzyme activity in plants by reversible phosphorylation (1988) Physiol Plant, 72, pp. 435-439
  • Cohen, P., Signal integration at the level of protein kinases, protein phosphatases and their substrates (1992) Trends Biochem Sci, 17, pp. 408-413
  • Cole, K.P., Blakeley, S.D., Dennis, D.T., Structure of the gene encoding potato cytosolic pyruvate kinase (1992) Gene, 122, pp. 255-261
  • Cyr, R.J., Palevitz, B.A., Organization of cortical microtubules in plant cells (1995) Curr Opin Cell Biol, 7, pp. 65-71
  • Estruch, J.J., Kadwell, S., Merlin, E., Crossland, L., Cloning and characterization of a maize pollen-specific calcium-dependent calmodulin-independent protein kinase (1994) Proc Natl Acad Sci USA, 91, pp. 8837-8841
  • Ferrell, J.E., Martin, G.S., Thrombin stimulates the activities of multiple previously unidentified protein kinases in platelets (1989) J Biol Chem, 264, pp. 20723-20729
  • Hannapel, D.J., Characterisation of the early events of potato tuber development (1991) Physiol Plant, 83, pp. 568-573
  • Harmon, A.C., Lee, J.-Y., Yoo, B.-C., Shao, J., Calcium and calcium-regulated protein kinases (1996) Plant Physiol, 111, pp. S-26. , abstract no. 61003
  • Harmon, A.C., Putnam-Evans, C., Cormier, M.J., A calcium-dependent but calmodulin-independent protein kinase from soybean (1987) Plant Physiol, 83, pp. 830-837
  • Hendriks, T., Vreugdenhil, D., Stiekema, J., Patatin and four serine protease inhibitor genes are differentially expressed during potato tuber development (1991) Plant Mol Biol, 17, pp. 385-394
  • Hidaka, H., Tanaka, T., Naphthalenesulfonamides as calmodulin antagonists (1983) Methods Enzymol, 102, pp. 185-194
  • Jena, P.K., Reddy, A.S., Poovaiah, B.W., Molecular cloning and sequencing of a cDNA for plant calmodulin: Signal-induced changes in the expression of calmodulin (1989) Proc Natl Acad Sci USA, 86, pp. 3644-3648
  • Kawasaki, T., Hayashida, N., Baba, T., Shinozaki, K., Shimada, H., The gene encoding a calcium-dependent protein kinase located near the sbe1 gene encoding starch branching enzyme I is specifically expressed in developing rice seeds (1993) Gene, 129, pp. 183-189
  • Koda, Y., Omer, E.-S.A., Yoshihara, T., Shibata, H., Sakamura, S., Okasawa, Y., Isolation of a tuber inducing substance from potato leaves (1988) Plant Cell Physiol, 29, pp. 1047-1051
  • Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
  • Leloir, L.F., Goldemberg, S.H., Synthesis of glycogen from uridine diphosphate glucose in liver (1960) J Biol Chem, 235, pp. 919-923
  • Lowry, O.H., Rosebrough, N.J., Farr, R.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275
  • Ma, H., Protein phosphorylation in plants: Enzymes, substrates and regulators (1993) Trends Genet, 9, pp. 228-230
  • Matsuki, T., Tazaki, H., Fujimori, T., Hogetsu, T., The influences of jasmonic acid methyl ester on microtubules in potato cells and formation of potato tubers (1992) Biosci Biotechnol Biochem, 56, pp. 1329-1330
  • Menzel, C.M., The control of storage organ formation in potato and other species: A review, part 1 (1985) Field Crops Abstracts, 38, pp. 527-537
  • Menzel, C.M., The control of storage organ formation in potato and other species: A review, part 2 (1985) Field Crops Abstracts, 38, pp. 581-606
  • Minichiello, J., Polya, G.M., Keane, P.J., Inhibition and activation of oat leaf calcium-dependent protein kinase by fatty acids (1989) Plant Sci, 65, pp. 143-152
  • Mizuno, K., Inhibition of gibberellin-induced elongation, reorientation of cortical microtubules and change of isoform of tubulin in epicotyl segments of azuki bean by protein kinase inhibitors (1994) Plant Cell Physiol, 35, pp. 1149-1157
  • Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures (1962) Physiol Plant, 15, pp. 473-497
  • Muto, S., Intracellular Ca2+ messenger system in plants (1992) Int Rev Cytol, 142, pp. 305-345
  • Ochatt, C.M., Ulloa, R.M., Tones, H.N., Téllez-Iñón, M.T., Characterization of the catalytic subunit of Trypanosoma cruzi cyclic AMP-dependent protein kinase (1993) Mol Biochem Parasitol, 57, pp. 73-78
  • Polya, G.M., Klucis, E., Haritou, M., Resolution and characterization of two soluble calcium-dependent protein kinases from silver beet leaves (1987) Biochim Biophys Acta, 931, pp. 68-77
  • Poovaiah, B.W., Reddy, A.S.N., Calcium and signal transduction in plants (1993) Crit Rev Plant Sci, 12, pp. 185-211
  • Pratt, L.H., McCurdy, D.W., Shimazaki, Y., Cordonnier, M.M., Immunodetection of phytocrome: Immunocytochemistry, immunoblotting and immunoquantitation (1986) Modern Methods of Plant Analysis, pp. 50-74. , HF Linskens, JF Jackson, eds, Springer-Verlag, New York
  • Putnam-Evans, C., Harmon, A.C., Cormier, M.J., Purification and characterization of a novel calcium-dependent protein kinase from soybean (1990) Biochemistry, 29, pp. 2488-2495
  • Roberts, D.M., Harmon, A.C., Calcium-modulated proteins: Targets of intracellular calcium signals in higher plants (1992) Annu Rev Plant Physiol Plant Mol Biol, 43, pp. 375-414
  • Roberts, D.M., Weaver, C.D., How plants respond to stimuli (1990) New Biologist, 2, pp. 678-683
  • Taylor, M.A., Davies, H.V., Scobie, L.A., Molecular changes associated with tuberisation in Solanum tuberosum: Differential expression of α-tubulin isotypes (1991) Physiol Plant, 81, pp. 244-250
  • Taylor, M.A., Mad Arif, S.A., Kumar, A., Davies, H.V., Scobie, L.A., Pearce, S.R., Flavell, A.J., Expression and sequence analysis of cDNAs induced during the early stages of tuberisation in different organs of the potato plant (Solanum tuberosum L.) (1992) Plant Mol Biol, 20, pp. 641-651
  • Téllez-Iñón, M.T., Ulloa, R.M., Glikin, G.C., Torres, H.N., Characterization of Neurospora crassa cyclic AMP phosphodiesterase activated by calmodulin (1985) Biochem J, 232, pp. 425-430
  • Tovar, P., Estrada, R., Schilde-Renschler, L., Dodds, J.H., Induction and use of in vitro tubers (1985) CIP Circular, 13, pp. 1-5
  • Ulloa, R.M., Glikin, G.C., Téllez-Iñón, M.T., Torres, H.N., Judewicz, N.D., A novel stimulator of protein phosphorylation in Neurospora crassa (1987) Mol Cell Biochem, 77, pp. 11-17
  • Ulloa, R.M., Mac Intosh, G., Melchiorre, M., Mentaberry, A.N., Dallari, P., Moriconi, D.N., Téllez-Iñón, M.T., Protein kinase activities in the different stages of potato (Solanum tuberosum L.) microtuberization (1996) Plant Cell Rep, , in press
  • Ulloa, R.M., Torres, H.N., Ochatt, C.M., Téllez-Iñón, M.T., Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa (1991) Mol Cell Biochem, 102, pp. 155-163
  • Vreugdenhil, D., Struik, P.C., An integrated view of the hormonal regulation of tuber formation in potato (Solanum tuberosum) (1989) Physiol Plant, 75, pp. 525-531
  • Yuasa, T., Muto, S., Ca2+-dependent protein kinase from the halotolerant green alga Dunaliella tertiolecta: Partial purification and Ca2+-dependent association of the enzyme to the microsomes (1992) Arch Biochem Biophys, 296, pp. 175-182

Citas:

---------- APA ----------
MacIntosh, G.C., Ulloa, R.M., Raíces, M. & Téllez-Iñón, M.T. (1996) . Changes in calcium-dependent protein kinase activity during in vitro tuberization in potato. Plant Physiology, 112(4), 1541-1550.
http://dx.doi.org/10.1104/pp.112.4.1541
---------- CHICAGO ----------
MacIntosh, G.C., Ulloa, R.M., Raíces, M., Téllez-Iñón, M.T. "Changes in calcium-dependent protein kinase activity during in vitro tuberization in potato" . Plant Physiology 112, no. 4 (1996) : 1541-1550.
http://dx.doi.org/10.1104/pp.112.4.1541
---------- MLA ----------
MacIntosh, G.C., Ulloa, R.M., Raíces, M., Téllez-Iñón, M.T. "Changes in calcium-dependent protein kinase activity during in vitro tuberization in potato" . Plant Physiology, vol. 112, no. 4, 1996, pp. 1541-1550.
http://dx.doi.org/10.1104/pp.112.4.1541
---------- VANCOUVER ----------
MacIntosh, G.C., Ulloa, R.M., Raíces, M., Téllez-Iñón, M.T. Changes in calcium-dependent protein kinase activity during in vitro tuberization in potato. PLANT PHYSIOL. 1996;112(4):1541-1550.
http://dx.doi.org/10.1104/pp.112.4.1541