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Abstract:

Temperature imaging based on the fluorescence of the complex [Ru(bpy) 3]2+ is described. The method allows precise temperature measurement on unidimensional flow injection reactors and bidimensional measurement on dishes for biological and biochemical assays. The fluorescence dependence on temperature is linear, achieving a resolution of 0.05 K with a simple two-point calibration. The large Stokes shift of [Ru(bpy) 3]2+ makes it easy to use a simple CCD camera without special filters. Large or small area fields can be achieved by changing the optics of the camera. High spatial resolution is possible by using any fluorescence microscope. © 2006 American Chemical Society.

Registro:

Documento: Artículo
Título:1D and 2D temperature imaging with a fluorescent ruthenium complex
Autor:Filevich, O.; Etchenique, R.
Filiación:Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA, Buenos Aires, Argentina
Palabras clave:Bioassay; Cameras; Chemical reactors; Fluorescence; Imaging techniques; Temperature measurement; Flow injection reactors; Fluorescence microscopes; Two-point calibration; Ruthenium compounds; ruthenium complex; article; bioassay; calibration; camera; filter; flow injection analysis; fluorescence; fluorescence microscope; imaging; linear system; optical resolution; optics; temperature imaging; temperature measurement
Año:2006
Volumen:78
Número:21
Página de inicio:7499
Página de fin:7503
DOI: http://dx.doi.org/10.1021/ac061382f
Título revista:Analytical Chemistry
Título revista abreviado:Anal. Chem.
ISSN:00032700
CODEN:ANCHA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v78_n21_p7499_Filevich

Referencias:

  • Price, P.B., Sowers, T., (2004) Proc. Natl. Acad. Sci. U.S.A., 101, pp. 4631-4636
  • Watzig, H., (1992) Chromatographia, 33, pp. 445-448
  • Otal, E.H., Iñón, F.A., Andrade, F.J., (2003) Applied Spectrosc., 57, pp. 661-666
  • Tallarek, U., Rapp, E., Scheenen, T., Bayer, E., Van As, H., (2000) Anal. Chem., 72, pp. 2292-2301
  • Lacey, M.E., Webb, A.G., Sweedler, J.V., (2000) Anal. Chem., 72, pp. 4991-4998
  • Sakakibara, J., Hishida, K., Maeda, M., (1993) Exp. Fluids, 16, pp. 82-96
  • Sakakibara, J., Adrian, R.J., (1999) Exp. Fluids, 26, pp. 7-15
  • Ross, D., Gaitan, M., Locascio, L.E., (2001) Anal. Chem., 73, pp. 4117-4123
  • Walker, G.W., Sundar, V.C., Rudzinski, C.M., Wun, A.W., Bawendi, M.G., Nocera, D.G., (2003) Appl. Phys. Lett., 83, pp. 3555-3557
  • Mao, H., Yang, T., Cremer, P.S., (2002) J. Am. Chem. Soc., 124, pp. 4432-4435
  • Biju, V., Makita, Y., Sonoda, A., Yokoyama, H., Baba, Y., Ishikawa, M., (2005) J. Phys. Chem, B, 109, pp. 13899-13905
  • Durham, B., Caspar, J.V., Nagle, J.K., Meyer, T.J., (1982) J. Am. Chem Soc., 104, pp. 4803-4810
  • Kolodner, P., Tyson, A., (1982) Appl. Phys. Lett, 40, pp. 782-784
  • Zohar, O., Ikeda, M., Shinagawa, H., Inoue, H., Nakamura, H., Elmaum, D., Alkon, D.L., Yoshioka, T., (1998) Biophys. J., 74, pp. 82-89
  • Van Houten, J., Watts, R.J., (1976) J. Am. Chem. Soc., 98, pp. 4853-4858
  • Bensasson, R., Salet, C., Balzani, V., (1976) J. Am. Chem. Soc., 98, pp. 3272-3273

Citas:

---------- APA ----------
Filevich, O. & Etchenique, R. (2006) . 1D and 2D temperature imaging with a fluorescent ruthenium complex. Analytical Chemistry, 78(21), 7499-7503.
http://dx.doi.org/10.1021/ac061382f
---------- CHICAGO ----------
Filevich, O., Etchenique, R. "1D and 2D temperature imaging with a fluorescent ruthenium complex" . Analytical Chemistry 78, no. 21 (2006) : 7499-7503.
http://dx.doi.org/10.1021/ac061382f
---------- MLA ----------
Filevich, O., Etchenique, R. "1D and 2D temperature imaging with a fluorescent ruthenium complex" . Analytical Chemistry, vol. 78, no. 21, 2006, pp. 7499-7503.
http://dx.doi.org/10.1021/ac061382f
---------- VANCOUVER ----------
Filevich, O., Etchenique, R. 1D and 2D temperature imaging with a fluorescent ruthenium complex. Anal. Chem. 2006;78(21):7499-7503.
http://dx.doi.org/10.1021/ac061382f